Expression of miRNAs in the Presence of HPV Infection in Cervical Dysplasia Samples: A Pilot Study

Authors

  • A. SVINTSITSKA Nonprofit organization “National Cancer Institute”, Kyiv, Ukraine
  • N. LYGYRDA Medical Center of Colposcopy “LyNa”, Kyiv, Ukraine
  • V. Svintsitskyi Nonprofit organization “National Cancer Institute”, Kyiv, Ukraine
  • T. Borikun R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the NASU, Kyiv, Ukraine
  • N. Lukianova R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the NASU, Kyiv, Ukraine

DOI:

https://doi.org/10.15407/exp-oncology.2024.04.387

Keywords:

cervical cancer, cervical dysplasia, HPV, miRNA

Abstract

Background. Cervical cancer is a major health concern, with human papillomaviruses (HPV) infection being a key risk factor. However, not all HPV-infected individuals develop cancer, suggesting the additional factors may be involved. This study aims to evaluate the differences in the miR-155 and -205 expression in cervical tissue with dysplasia depending on the presence of HPV and confirmed cancer diagnosis. Materials and Methods. The expression of miR-155 and -205 in 30 formalin-fixed paraffin-embedded primary cervical tissue biopsy samples was evaluated using RT-PCR. Results. The expression levels of miRNA-155 and -205 in cervical dysplasia samples without malignant transformation was lower than these in carcinoma in situ tissues (0.74 ± 0.21 and 1.65 ± 0.42 vs. 1.37 ± 0.18 and 2.35 ± 0.32, respectively). In carcinoma in situ cases, we found higher levels of miRNA-155 and -205 (1.6 and 1.38 times, respectively) in CIN-3/ HSIL samples compared to CIN-2/HSIL samples. The expression of both miRNAs tended to increase in HPV-positive cases and in the presence of malignant transformation compared to HPV-negative dysplasia and dysplasia without signs of malignant transformation, respectively. Conclusions. The obtained data indicate a potential relationship between the presence of HPV infection and the expression profile of miRNA-155 and -205.

References

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Published

20.02.2025

How to Cite

SVINTSITSKA, A., LYGYRDA, N., Svintsitskyi, V., Borikun, T., & Lukianova, N. (2025). Expression of miRNAs in the Presence of HPV Infection in Cervical Dysplasia Samples: A Pilot Study. Experimental Oncology, 46(4), 387–392. https://doi.org/10.15407/exp-oncology.2024.04.387

Issue

Vol. 46 No. 4 (2024): Experimental Oncology

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Original contributions

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