Differential Expression of MRPS18 Family Genes in Brain Tumor Samples

Authors

  • L. KOVALEVSKA R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU, Kyiv, Ukraine
  • S. KALMAN R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU, Kyiv, Ukraine
  • A. SUSHNOVA R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU, Kyiv, Ukraine
  • O. MALYSHEVA The State Institution A.P. Romodanov Neurosurgery Institute of NAMSU, Kyiv, Ukraine
  • V. ROZUMENKO The State Institution A.P. Romodanov Neurosurgery Institute of NAMSU, Kyiv, Ukraine
  • T. MALYSHEVA The State Institution A.P. Romodanov Neurosurgery Institute of NAMSU, Kyiv, Ukraine
  • E. KASHUBA R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU, Kyiv, Ukraine

DOI:

https://doi.org/10.15407/exp-oncology.2024.04.368

Keywords:

MRPS18 family proteins, brain tumors, expression pattern

Abstract

Background. Brain tumors account for 2%—3% of all malignant neoplasms and 85%—90% of all primary tumors of the central nervous system with the 5-year survival rate of 35%. Additional biomarkers could help refine the molecular profile of brain tumors and prognosis of the disease. Aim. To study differential expression patterns of the MRPS18 family genes in tumor tissue and the peripheral blood of patients with brain tumors of various types. Materials and Methods. The total RNA was isolated from blood and tumor tissue samples of 27 patients with brain tumors. The quantitative polymerase chain reaction (qPCR) was performed. Also, immunohistochemical (IHC) studies of the MRPS18 family proteins were performed on deparaffinized tissue sections. Results. The MRPS18-1-3 genes were highly expressed at the mRNA level in tumor tissue and the peripheral blood of patients with brain tumors. All 3 genes showed different patterns of expression depending on the tumor type. The highest MRPS18-1 mRNA expression was detected in glioblastoma (GB) samples in both tumor samples and the peripheral blood. In general, MRPS18-1 expression was higher in G4 tumors, compared to G2. MRPS18-3 gene was expressed as higher levels in G2 samples and in embryonic tumors. MRPS18-2 was expressed in all studied samples, with no regard to the tumor grade or type. The MRPS18-2 IHC staining was detected at high levels in most brain tumors. Conclusions. The MRPS18 family genes showed similar patterns of mRNA expression in tissue samples of brain tumors and peripheral blood of patients. The highest levels of MRPS18-1 mRNA were detected in GB samples, while the highest protein signal was detected for MRPS18-2 in almost all brain tumor samples.

References

Satgunaseelan L, Sy J, Shivalingam B, et al. Prognostic and predictive biomarkers in central nervous system tumours: the molecular state of play. Pathology. 2024;56(2):158-169. https://doi.org/10.1016/j.pathol.2023.11.005

Malbari F, Lindsay H. Genetics of common pediatric brain tumors. Pediatr Neurol. 2020;104:3-12. https://doi. org/10.1016/j.pediatrneurol.2019.09.007

Louis DN, Perry A, Wesseling P, et al. The 2021 WHO Classification of Tumors of the Central Nervous System: a summary. Neuro-oncology. 2021;23(8):1231-1251. https://doi.org/10.1093/neuonc/noab106

Han PC, Baker TG. Glial and glioneuronal tumors: Navigating the complexity of evolving concepts and new clas- sification. J Neurol Sci. 2024;461:123058. https://doi.org/10.1016/j.jns.2023.123058

Reznicek J, Sharifai N, Jamshidi P, et al. Embryonal and pineal tumours. Cytopathology. 2024;35(5):561-571. https:// doi.org/10.1111/cyt.13123

Behnan J, Finocchiaro G, Hanna G. The landscape of the mesenchymal signature in brain tumours. Brain. 2019;142(4):847-866. https://doi.org/10.1093/brain/awz044

Packer RJ, Pfister S, Bouffet E, et al. Pediatric low-grade gliomas: implications of the biologic era. Neuro-oncology. 2017;19(6):750-761. https://doi.org/10.1093/neuonc/now209

Jiang J, Wang S, Chen Y, et al. Immunohistochemical characterization of lymphangiogenesis-related biomarkers in primary and recurrent gliomas: A STROBE compliant article. Medicine. 2018;97(39):e12458. https://doi. org/10.1097/MD.0000000000012458

Shcherbina V, Kovalevska L, Pedachenko E, et al. Comparative analysis of the embryonal brain tumors based on their molecular features. Discov Med. 2023;35(178):733-749. https://doi: 10.24976/Discov.Med.202335178.69

Raffel C. Medulloblastoma: molecular genetics and animal models. Neoplasia. 2004;6(4):310-322. https://doi. org/10.1593/neo.03457

Udaka YT, Packer RJ. Pediatric brain tumors. Neurol Clin. 2018;36(3):533-556. https://doi.org/10.1016/j. ncl.2018.04.001

Swartling FJ, Bolin S, Phillips JJ, Persson AI. Signals that regulate the oncogenic fate of neural stem cells and progeni- tors. Exp Neurol. 2014;260:56-68. https://doi.org/10.1016/j.expneurol.2013.11.010

Kovalevska L, Matveeva A, Kashuba E. A role of the RB protein in the support of cell stemness. Oncology. 2023;25(4):252-261. https://doi.org/10.15407/oncology.2023.04.245

Mushtaq M, Gaza HV, Kashuba EV. Role of the RB-interacting proteins in stem cell biology. Adv Cancer Res. 2016;131:133-157. https://doi.org/10.1016/bs.acr.2016.03.001

Kashuba E, Mushtaq M. Do MRPS18-2 and RB proteins cooperate to control cell stemness and differentiation, pre- venting cancer development? Exp Oncol. 2017;39(1):12-16.

Mushtaq M, Kovalevska L, Darekar S, et al. Cell stemness is maintained upon concurrent expression of RB and the mi- tochondrial ribosomal protein S18-2. Proc Natl Acad Sci U S A. 2020;117(27):15673-15683. https://doi.org/10.1073/ pnas.2002432117

Mushtaq M, Ali RH, Kashuba V, et al. S18 family of mitochondrial ribosomal proteins: evolutionary history and Gly132 polymorphism in colon carcinoma. Oncotarget. 2016;7(34):55649-55662. https://doi.org/10.18632/on- cotarget.10595

Kovalevska LM, Malysheva TA, Kalman SS, et al. Expression pattern of MRPS18 family genes in gliomas. Exp Oncol.

;43(3):204-208. https://doi.org/10.32471/exp-oncology.2312-8852.vol-43-no-3.16461

Kovalevska L, Kashuba E. Role of MRPS18 family in malignant cell transformation (UKR). In: Chekhun VF, edi- tor. The ways and perspective of development of experimental oncology in Ukraine (Shljahy ta perspektyvy rozvytku eksperimentalnoi onkologii v Ukraini). Kyiv, Ukraine: DIA; 2021: 77-90.

Kovalevska LM, Shcherbina VM, Kalman SS, et al. Expression pattern of MRPS18 family genes in medulloblastoma: a case report. Ukr Neurosurg J. 2022;28(4):35-40. https://doi.org/10.25305/unj.265302

Sorensen KM, Meldgaard T, Melchjorsen CJ, et al. Upregulation of Mrps18a in breast cancer identified by selecting phage antibody libraries on breast tissue sections. BMC Cancer. 2017;17(1):19. https://doi.org/10.1186/s12885-016- 2987-5

Lin X, Guo L, Lin X, et al. Expression and prognosis analysis of mitochondrial ribosomal protein family in breast cancer. Sci Rep. 2022;12(1):10658. https://doi.org/10.1038/s41598-022-14980-0

Kovalevska LM, Zadvornyi TV, Malysheva TA, et al. Study on relative gene expression levels in tumor tissue and in blood serum of cancer patients. Oncology (Onkologia). 2021;23(3):149-153. https://doi.org/10.32471/ oncology.2663-7928.t-23-3-2021-g.9761 (in Ukrainian).

Downloads

Published

20.02.2025

How to Cite

KOVALEVSKA, L., KALMAN, S., SUSHNOVA, A., MALYSHEVA, O., ROZUMENKO, V., MALYSHEVA, T., & KASHUBA, E. (2025). Differential Expression of MRPS18 Family Genes in Brain Tumor Samples. Experimental Oncology, 46(4), 368–374. https://doi.org/10.15407/exp-oncology.2024.04.368

Issue

Section

Original contributions

Most read articles by the same author(s)