Study of modifying effects of astaxanthin on cytogenetic manifestations of bystander response in human peripheral blood lymphocytes in vitro
DOI:
https://doi.org/10.32471/exp-oncology.2312-8852.vol-43-no-2.16301Keywords:
137Cs γ-quanta, astaxanthin, B-cell CLL, chromatid-type aberrations, chromosome instability, chromosome-type aberrations, RIBE, TIBEAbstract
Summary. Aim: To study the possible impact of astaxanthin on the cytogenetic manifestations of the simultaneous development of radiation-induced (RIBE) and tumor-induced bystander effect (TIBE) in human peripheral blood lymphocytes. Materials and Methods: Peripheral blood lymphocytes from healthy persons and patients with chronic lymphocytic leukemia were cultured separately or cocultured with or without previous irradiation in vitro by 137Cs at a dose of 0.5 Gy. The cells were cultured with and without 20 μg/ml astaxanthin. Results: In the presence of astaxanthin, the decrease of chromosomal instability both in the variant with separate TIBE and with simultaneous development of TIBE and RIBE was observed as the reduction in the frequency of simple chromosome-type aberrations, namely, double fragments. The average level of chromatid-type aberrations did not change under the action of astaxanthin. Although the total chromosome instability in bystander cells diminished, this did not lead to the elimination of the RIBE and TIBE development in the presence of astaxanthin. Conclusion: In the setting of experiment, astaxanthin did not reduce the frequency of chromatid-type aberrations in bystander cells due to RIBE and TIBE but reduced the frequency of simple aberrations of chromosomal type, not associated with the development of bystander response phenomenon.
References
Verma N, Tiku AB. Significance and nature of bystander responses induced by various agents. Mutat Res 2017; 773: 104–21.
Burdak-Rothkamm S, Rothkamm K. Radiation-induced bystander and systemic effects serve as a unifying model system for genotoxic stress responses. Mutat Res 2018; 778: 13–22.
Shemetun OV, Pilinska MA. Radiation-induced bystander effect — modeling, manifestation, mechanisms, persistence, cancer risks (literature review). Probl Radiac Med Radiobiol 2019; 24: 65–92.
Djomina EA. Early and late radiation effects in healthy tissues of oncologic patients under therapeutic irradiations Probl Radiac Med Radiobiol 2017; 22: 23–37.
Wang R, Zhou T, Liu W, Zuo L. Molecular mechanism of bystander effects and related abscopal/cohort effects in cancer therapy. Oncotarget 2018; 9: 18637–47.
He X, Wu W, Ding Y, et al. Excessive risk of second primary cancers in young-onset colorectal cancer survivors. Cancer Med 2018; 7: 1201–10.
Pilinska MA, Shemetun OV, Talan OA, et al. Study the effects of ionizing radiation on the level of chromosome instability in human somatic cells during the development of tumor-induced bystander effect. Probl Radiac Med Radiobiol 2020; 25: 313–21.
Pilinska MA, Shemetun OV. Investigation of certain manifestations of bystander response phenomenon that may affect the development of human cancer pathology. Biomed J Sci Tech Res 2021; 33: 25986–7.
Djomina EA. Antiradiation means: classification and mechanisms. Probl Radiac Med Radiobiol 2015; 20: 42–54 (in Russian).
Domina EA. Experience on using radiomitigators in radiation therapy of 245 cancer patients. J Sci 2020; 1: 7–11.
Mosse IB, Morozyk PM. Radio-induced «bystander» effect and its biological meaning. Scientific works. Tech Safety 2010; 139: 37–41.
Konopacka M, Rzeszowska-Wolny J. The bystander effect-induced formation of micronucleated cells is inhibited by antioxidants, but the parallel induction of apoptosis and loss of viability are not affected. Mutat Res 2006; 593: 32–8.
Konopacka M. The influence of antioxidant vitamins on the radiation-induced bystander effect in normal human lymphocytes. Modern problems of radiation research. 35th Annual Conference of the European Society for Radiological Research. Сonference proceedings. K. 2007: 94–101.
Shemetun OV. Modification of radiation-induced cytogenetic effects in human peripheral blood lymphocytes using antioxidant vitaminic medicinal product. Dop Nac Acad Nauk Ukr 2013; 9: 191–5 (in Ukrainian).
Shemetun OV, Talan OO. Investigation of modification of X-ray induced bystander effect in vitro. Probl Radiac Med Radiobiol 2014; 19: 371–6 (in Ukrainian).
Olivares A, Alcaraz-Saura M, Achel DG, et al. Radiation-induced bystander effect: Loss of radioprotective capacity of rosmarinic acid in vivo and in vitro. Antioxidants 2021; 10: 231–9.
Kurinnyi DA, Rushkovskyi SR, Demchenko OM, et al. Comparison of the modifying action of asthaxantin on the development of radiation-induced chromosome instability in human blood lymphocytes irradiated in vitro at different cell cycle stages. Cytol Genet 2018; 52: 368–73.
Kurinnyi DA, Rushkovskyi SR, Demchenko OM, Pilinska MA. Characteristics of the effects of astaxanthin on radiation-induced bystander effect realization in human peripheral blood lymphocytes. J Nat Acad Med Nauk Ukr 2019; 25: 133–8 (in Ukrainian).
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