Clinicopathological correlation of the expression of excision repair cross complementation group 1 (ERCC1) in oral cavity squamous cell carcinomas: an immunohistochemical study
DOI:
https://doi.org/10.32471/exp-oncology.2312-8852.vol-42-no-2.14461Keywords:
DNA damage, ERCC1, nuclear expression, nucleotide excision repair, oral cancerAbstract
Summary. Background: The nucleotide excision repair pathway is a sophisticated DNA repair mechanism that reduces DNA damage caused by exogenous factors. Excision repair cross complementation group 1 (ERCC1) is a prominent member of this pathway that maintains the genomic stability. The aim of this study is to determine the association between the immunohistochemical expression of ERCC1 and the clinical and pathological features of oral cavity squamous cell carcinomas. Materials and Methods: The sections of formalin fixed paraffin embedded tissue blocks of oral squamous cell carcinomas (n = 60) were immunohistochemically stained with anti-ERCC1 antibody. The association between the nuclear expression of ERCC1 and the clinicopathological parameters of the tumors and the patient outcomes was evaluated using the chi-square test. Results: ERCC1 expression was evident in all studied cases of the oral squamous cell carcinomas. A high ERCC1 expression was associated with smaller tumors, tumors without lymph node involvement and well-differentiated tumors (p < 0.001). Better outcomes were associated with higher expression of ERCC1 (p = 0.028). Conclusion: ERCC1 seems to be an efficient biomarker for prognostication of oral squamous cell carcinomas. High expression of ERCC1 indicates more favorable course of the disease.
References
Day TA, Davis BK, Gillespie MB, et al. Oral cancer treatment. Curr Treat Options Oncol 2003; 4: 27–41.
Varshitha A. Prevalence of oral cancer in India. J Pharm Sci Res 2015; 7: 845–8.
Sankaranarayanan R, Ramadas K, Thomas G, et al. Effect of screening on oral cancer mortality in Kerala, India: a cluster-randomised controlled trial. Lancet 2005; 365: 1927–33.
Gupta PC, Nandakumar A. Oral cancer scene in India. Oral Dis 1999; 5: 1–2.
Forastiere A, Koch W, Trotti A, et al. Head and neck cancer. N Engl J Med 2001; 345: 1890–900.
Agarwal AK, Sethi A, Sareen D, et al. Treatment delay in oral and oropharyngeal cancer in our population: the role of socio-economic factors and health-seeking behaviour. Ind J Otolaryngol Head Neck Surg 2011; 63: 145–50.
Freier K, Joos S, Flechtenmacher C, et al. Tissue microarray analysis reveals site-specific prevalence of oncogene amplifications in head and neck squamous cell carcinoma. Cancer Res 2003; 63: 1179–82.
Cadet J, Wagner JR. DNA base damage by reactive oxygen species, oxidizing agents, and UV radiation. Cold Spring Harb Perspect Biol 2013; 5: 1–18.
Altieri F, Grillo C, Maceroni M, et al. DNA damage and repair: from molecular mechanisms to health implications. Antioxid Redox Signal 2008; 10: 891–937.
DeLaat W, Jaspers N, Hoeijmakers J. Molecular mechanism of nucleotide excision repair. Genes Dev 1999; 13: 768–85.
Friboulet L, Vinay SP, Sourisseau T. ERCC1 function in nuclear excision and interstrand crosslink repair pathways is mediated exclusively by the ERCC1-202 isoform. Cell Cycle 2013; 12: 3298–306.
Jian-Wei B, Yi-Min M, Yu-Xia S, et al. Expression levels of ERCC1 and RRM1 mRNA and clinical outcome of advanced non-small cell lung cancer. Pak J Med Sci 2013; 29: 1158–61.
Gerhard R, Carvalho A, Carneiro V, et al. Clinicopathological significance of ERCC1 expression in breast cancer. Pathol Res Pract 2013; 209: 331–6.
Yuanming L, Lineng Z, Baorong S. BRCA1 and ERCC1 mRNA levels are associated with lymph node metastasis in chinese patients with colorectal cancer. BMC Cancer 2013; 13: 103.
Joshi MB, Shirota Y, Danenberg KD, et al. High gene expression of TS1, GSTP1, and ERCC1 are risk factors for survival in patients treated with trimodality therapy for esophageal cancer. Clin Cancer Res 2005; 11: 2215–21.
Dabholkar M, Vionnet J, Bostick-Bruton F, et al. Messenger RNA levels of XPAC and ERCC1 in ovarian cancer tissue correlate with response to platinum-based chemotherapy. J Clin Invest 1994; 94: 703–8.
Bajpai D, Banerjee A, Pathak S. Decreased expression of DNA repair genes (XRCC1, ERCC1, ERCC2, and ERCC4) in squamous intraepithelial lesion and invasive squamous cell carcinoma of the cervix. Mol Cell Biochem 2013; 377: 45–53.
Jun HJ, Ahn MJ, Kim HS, et al. ERCC1 expression as a predictive marker of squamous cell carcinoma of the head and neck treated with cisplatin-based concurrent chemoradiation. Br J Cancer 2008; 99: 167–72.
Chiu TJ, Chen CH, Chien CY, et al. High ERCC1 expression predicts cisplatin-based chemotherapy resistance and poor outcome in unresectable squamous cell carcinoma of head and neck in a betel-chewing area. J Transl Med 2011; 9: 31.
Hayes M, Lan C, Yan J, et al. ERCC1 expression and outcomes in Head and Neck cancer treated with concurrent cisplantin and radiation. Anticancer Res 2011; 31: 4135–40.
Gregg SQ, Robinson AR, Niedernhofer LJ. Physiological consequences of defects in ERCC1-XPF DNA repair endonuclease. DNA Repair (Amst) 2011; 10: 781–91.
Manjari M, Popli R, Paul S, et al. Prevalence of oral cavity, pharynx, larynx and nasal cavity malignancies in Amritsar, Punjab. Indian J Otolaryngol Head Neck Surg 1996; 48: 191–5.
Kalogeraki A, Karvela-Kalogeraki I, Tamiolakis D. ERCC1 expression correlated with EGFR and clinicopathological variables in patients with non-small cell lung cancer. An immunocytochemical study on fine-needle aspiration biopsies samples. Rev Port Pneumol 2014; 20: 200–7.
Shen MR, Jones IM, Mohrenweiser H. Non conservative amino acid substitution variants exists at polymorphic frequency in DNA repair genes in healthy humans. Cancer Res 1998; 58: 604–7.
Quintela-Fandino M, Hitt R, Medina PP, et al. DNA-repair Gene polymorphisms predict favourable clinical outcome among patients with advanced squamous cell carcinoma of the head and neck treated with cisplatin-based induction chemotherapy. J Clin Oncol 2006; 24: 4333–9.
Bilen N, Tekin SB, Topdagi O. ERCC1 expression in non-small cell lung and esophageal cancer. Eurasian J Med 2014; 46: 84–8.
An HJ, Jo H, Jung CK, et al. Prognostic implication of ERCC1 protein expression in resected oropharynx and oral cavity cancer. Pathol Res Pract 2017; 213: 949–55.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Experimental Oncology
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
