RED RICE BRAN EXTRACT SUPPRESSES COLON CANCER CELLS VIA APOPTOSIS INDUCTION/CELL CYCLE ARREST AND EXERTS ANTIMUTAGENIC ACTIVITY

Authors

  • Ratsada Praphasawat Department of Pathology, School of Medicine, University of Phayao, Phayao, Thailand 56000
  • Sarawoot Palipoch School of Medicine, Walailak University, Nakhon Si Thammarat, Thailand 80160
  • Prasit Suwannalert Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand 10400
  • Witchuda Payuhakrit Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand 10400
  • Paween Kunsorn Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand 10400
  • Sarunya Laovitthayanggoon Expert Centre of Innovative Herbal Products, Thailand Institute of Scientific and Technological Research, Techno Polis, Khlong Luang District, Pathum Thani, Thailand 12120
  • Sinittra Thakaew Department of Pathology, School of Medicine, University of Phayao, Phayao, Thailand 56000
  • Narongsuk Munkong Department of Pathology, School of Medicine, University of Phayao, Phayao, Thailand 56000
  • Warangkhana Klajing Department of Traditional Chinese Medicine, School of Public Health, University of Phayao, Phayao, Thailand 56000

DOI:

https://doi.org/10.15407/exp-oncology.2023.02.220

Keywords:

colon cancer cells, apoptosis, cell cycle arrest, antimutagenicity, red rice bran extract

Abstract

Background. Red rice bran extract (RRBE) contains many biologically active substances exerting antioxidant and anti-inflammatory effects. Aim. To evaluate the anticancer potential of RRBE in human colon cancer cells and its mutagenic/antimutagenic effects on nonmalignant cells. Materials and Methods. The cytotoxic effect of RRBE was determined by trypan blue exclusion in HCT116, HT29 cell lines and a non-cancerous HEK293 cell line, and its antiproliferative effect using MTS and colony formation assay. The apoptosis induction was evaluated using ELISA, and the apoptotic rate and cell cycle progression were assessed by flow cytometry. The mutagenic/ antimutagenic potential of RRBE was analyzed by micronucleus assay in the V79 cell line. Results. RRBE caused a dose-dependent reduction of cell viability in colon cancer cells and showed a limited cytotoxicity against HEK293 cells. The treatment with RRBE suppressed proliferation of HCT116 and HT29 cells and induced apoptosis as evidenced by the increased DNA fragmentation and the apoptotic cell counts. Furthermore, RRBE treatment significantly increased the number of cells at the G2/M phase triggering the arrest of the cell cycle in colon cancer cells. Interestingly, RRBE did not increase the micronucleus frequency in V79 cells but reduced the micronucleus formation caused by mitomycin C. Conclusion. RRBE effectively suppressed proliferation, induced apoptosis, and caused a cell cycle arrest in human colon cancer cells while being non-mutagenic and exerting antimutagenic effects in vitro.

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Submitted: October 03, 2022

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Published

11.10.2023

How to Cite

Praphasawat, R., Palipoch, S., Suwannalert, P., Payuhakrit, W., Kunsorn, P., Laovitthayanggoon, S., … Klajing, W. (2023). RED RICE BRAN EXTRACT SUPPRESSES COLON CANCER CELLS VIA APOPTOSIS INDUCTION/CELL CYCLE ARREST AND EXERTS ANTIMUTAGENIC ACTIVITY. Experimental Oncology, 45(2), 220–230. https://doi.org/10.15407/exp-oncology.2023.02.220

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Vol. 45 No. 2 (2023): Experimental Oncology

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