Redox dependent features of tumors, adipose tissue, neutrophiles and platelets in patients with metastatic colorectal cancer


  • A.P. Burlaka R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology
  • S.V. Virko V.E. Lashkaryov Institute of Semiconductor Physics
  • A.A. Burlaka National Cancer Institute, Ministry of Health of Ukraine, Kyiv 03022, Ukraine
  • K.L. Krupnyk R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology



colorectal cancer., free radical generation, neutrophils, NO, obesity, platelets


Summary. Aim: To study the levels of redox-forming molecules generated in tumor, adipose tissue (AT), neutrophils and platelets of patients with colorectal cancer (CRC) with normal body weight or obesity. Materials and Methods: 83 samples of tumor tissue and 83 samples of the AT taken at the distance of 5 cm from the tumor as well as 83 blood samples have been analyzed in the patients with CRC with metachronous lesions in liver. The comparison group consisted of 18 patients who were surgically treated for ulcer. The rates of free radical generation were measured by electron paramagnetic resonance. Results: Free radical generation rate in tumor tissue and AT increased and nitrogen oxide (NO) levels decreased in patients with body mass index ≥ 25 kg/m2 as compared to the patients with normal body weight (p < 0.05). The superoxide generation activity in neutrophils of CRC patients exceeded about 7-fold the values obtained in neutrophils from patients with ulcer while the NO levels produced by neutrophils of CRC patients decreased significantly (p < 0.01). The same trends held true for the platelets. Conclusions: In obese patients with CRC, superoxide-generating activity increases in cancer cells, AT, neutrophils and platelets. The obesity should be considered as the additional oncological risk representing the potent predictor for the events realized in obese patients such as oxidative stress, inflammation, insulin resistance and endothelial dysfunction.


Sreevalsan S, Safe S. Reactive oxygen species and colorectal cancer. Curr Colorectal Cancer Rep 2013; 9: 350–7.

Aggarwal V, Tuli HS, Varol A, et al. Role of reactive oxygen species in cancer progression: Molecular mechanisms and recent advancements. Biomolecules 2019; 9: 735.

Helms C, Kim-Shapiro DB. Hemoglobin-mediated nitric oxide signaling. Free Radic Biol Med 2013; 61: 464–72.

Burlaka AP, Sydoryk YeP. [Radical forms of oxygen and nitric oxide in tumor process] Kyiv: Naukova Dumka, 2006. 228 p. (in Russian).

Matsubara C, Nishikawa Y, Yoshida Y, Takamura K. A spectrophotometric method for the determination of free fatty acid in serum using acyl-coenzyme A synthetase and acyl-coenzyme A oxidase. Аnal Biochem 1983; 130: 128–33.

Вurlaka AP, Burlaka AA, Virko SV, Ganusevich II. Molecular mechanisms of oxidation damage and liver cell dysfunction in patients with metastatic colorectal cancer. Exp Oncol 2019; 41: 328–34. 2312-8852.vol-41-no-4.13796.

McMurray F, Patten DA, Harper M-E. Reactive oxygen species and oxidative stress in obesity — recent findings and empirical approaches. Obesity (Silver Spring) 2016; 24: 2301–10.

Ataie Z, Dastjerdi M, Farrokhfall K, Ghiravani Z. The effect of cinnamaldehyde on iNOS activity and NO-induced islet insulin secretion in high-fat-diet rats. Evid Based Complement Alternat Med 2021; 2021: 9970678.

Suresh V, Reddy A. Dysregulation of nitric oxide synthases during early and late pathophysiological conditions of diabetes mellitus leads to amassing of microvascular impedement. J Diabetes Metab Disord 2021; 20: 989–1002.

Avci E, Karabulut A, Alp AG, et al. Crucial markers showing the risk of coronary artery disease in obesity: ADMA and neopterin. J Med Biochem 2020 39: 452–9.

Hiramatsu S, Tanaka H, Nishimura J, et al. Gastric cancer cells alter the immunosuppressive function of neutrophils. Oncol Rep 2020; 43: 251–9.

Tsuhako R, Yoshida H, Sugita C, Kurokawa M. Naringenin suppresses neutrophil infiltration into adipose tissue in high-fat diet-induced obese mice. J Nat Med 2020; 74: 229–37.

Mensurado S, Rei M, Lança T, et al. Tumor-associated neutrophils suppress pro-tumoral IL-17+ γδT cells through induction of oxidative stress. PLoS Biol 2018; 16: e2004990.

Wachowicz B, Olas B, Zbikowska H, Buczyński A. Generation of reactive oxygen species in blood platelets. Platelets 2002; 13: 175–82.

Lin MS, Huang JX, Zhu J, Shen HZ. Elevation of platelet count in patients with colorectal cancer predicts tendency to metastases and poor prognosis. Hepatogastroenterology 2012; 59: 1687–90.

Federico A, Morgillo F, Tuccillo C, et al. Chronic inflammation and oxidative stress in human carcinogenesis. Int J Cancer 2007; 121: 2381–6.




How to Cite

Burlaka, A., Virko, S., Burlaka, A., & Krupnyk, K. (2023). Redox dependent features of tumors, adipose tissue, neutrophiles and platelets in patients with metastatic colorectal cancer. Experimental Oncology, 43(3), 261–265.



Original contributions

Most read articles by the same author(s)

<< < 1 2