Expression of TLR4 and major inflammatory cytokines in patients with bladder cancer of different grade and stage

Authors

  • P.G. Yakovlev Clinical Hospital “Feofaniya”, Center of Urology and Oncourology, Kyiv 03143, Ukraine
  • O.I. Gorbach National Cancer Institute, Kyiv 03022, Ukraine
  • L.I. Ostapchenko Taras Shevchenko National University of Kyiv
  • L.V. Garmanchuk Taras Shevchenko National University of Kyiv
  • O.V. Skachkova National Cancer Institute, Kyiv 03022, Ukraine
  • N.M. Khranovska National Cancer Institute, Kyiv 03022, Ukraine
  • N.V. Senchylo Taras Shevchenko National University of Kyiv

DOI:

https://doi.org/10.32471/exp-oncology.2312-8852.vol-43-no-2.16102

Keywords:

gene expression, INF-γ, TGF-β1, TLR4, TNF, urinary bladder cancer

Abstract

Summary. Background: The bladder cancer is immunogenic, and neoantigens generated by tumor cells trigger a notable immune response in the host. On the other hand, multiple immune escape mechanisms allow for avoiding the recognition by the host immune system. Toll-like receptor type 4 and inflammatory cytokines play major role in the immune response to bladder cancer. Aim: To assess the expression of TLR4 and the genes of major inflammatory cytokines in tumor cells and in unaffected tissue of the bladder. Materials and Methods: The pairs of samples from the urinary bladder tumor and unaffected adjacent tissue were obtained from 50 surgically treated patients with bladder cancer. The level of expression of TLR4, TGF-β1, INF-γ, TNF-α genes was evaluated by real-time polymerase chain reaction. Results: Bladder cancer cells are characterized by lower expression levels of TLR4, TGF-β1, INF-γ, TNF-α as compared to unaffected tissue. In patients with recurrent cancer, expression of TLR-4 and cytokines does not change both in tumor and in unaffected tissue of the bladder. Expression of TLR4 is identically low both in low- and high-grade cancer. Expression levels of the INF-γ and TNF-α are remarkably low in muscle-invasive cancer compared to the unaffected bladder tissue. The level of TGF-β1 in bladder cancer is comparable to the unaffected tissue of the bladder, while in the intact and metastatic lymph nodes it is significantly upregulated. Conclusion: Bladder cancer tissue differs from the unaffected part of the bladder wall in the level of TLR4, TGF-β1, INF-γ, TNF-α expression.

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Published

26.05.2023

How to Cite

Yakovlev, P., Gorbach, O., Ostapchenko, L., Garmanchuk, L., Skachkova, O., Khranovska, N., & Senchylo, N. (2023). Expression of TLR4 and major inflammatory cytokines in patients with bladder cancer of different grade and stage. Experimental Oncology, 43(2), 142–148. https://doi.org/10.32471/exp-oncology.2312-8852.vol-43-no-2.16102

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