Application of gold and silver nanoparticles for selective assay of spermine in mixture with spermidine


  • Yu.V. Yanish R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology
  • S.P. Zaletok R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology
  • N.V. Vityuk Chuiko Institute of Surface Chemistry
  • Yu.P. Mukha Chuiko Institute of Surface Chemistry



colloid, gold, spectrophotometry, spermidine, spermine


Aim: To assess the applicability of the novel technique based on the detection of spermine in solutions by spectrocolorimetric method using gold and silver colloidal nanoparticles. Materials and Methods: Colloidal solution of gold nanoparticles were synthesized by chemical reduction of tetrachlorauric acid with trisodium citrate. Colloidal solution of silver nanoparticles was obtained by chemical reduction of silver nitrate with tryptophan. The absorption spectra of gold/silver metal colloids and their mixtures with polyamines were recorded. Results: The increase of spermine concentration in solution caused the change in the intensity of the band of localized surface plasmon resonance that was not affected by the excess of spermidine. The color shift in colloidal gold due to its aggregation with spermine was registered spectrophotometrically. Conclusion: The principal possibility of selective quantification of spermine in the presence of spermidine in extremely high concentration using colloidal gold has been shown. This method can be used to assay selectively spermine in biological fluids.


Fletcer JT, Bruck BS. Spermine detection via metal-mediated ethynylarene “turn-on” fluorescence signaling. Sens Actuators B Chem 2015; 207: 843–8.

Jornet-Martínez N, González-Béjar M, Moliner-Martínez Y, et al. Sensitive and selective plasmonic assay for spermine as biomarker in human urine. Anal Chem 2014; 86: 1347–51.

Kimling J, Maier M, Okenve B, et al. Turkevich method for gold nanoparticle synthesis revisited. J Phys Chem B 2006; 110: 15700–7.

Mukha IP, Eremenko AM, Smirnova NP, et al. Antimicrobial activity of stable silver nanoparticles of a certain size. Appl Biochem Microbiol 2013; 49: 199–206.

Bogatyrov VM, Gun’ko VM, Galaburda MV, et al. The effect of photoactivated transformations of Ag+ and Ag0 in silica fillers on their biocidal activity. Res Chem Intermed 2019; 45: 3985–4001.

Katifelis H, Lyberopoulou A, Mukha Iu, et al. Ag/Au bimetallic nanoparticles induce apoptosis in human cancer cell lines via P53, CASPASE-3 and BAX/BCL-2 pathways. Artif Cells Nanomed Biotechnol 2018; 46 (S3): S389–98.

Zaletok SP, Klenov OO, Gogol SV, et al. Blood and urine polyamines as new diagnostic markers of prostate cancer. Oncologiya 2019; 21: 219–23 (in Ukrainian).

Tsoi TH, Chan CF, Chan WL, et al. Urinary polyamines: a pilot study on their roles as prostate cancer detection biomarkers. PLOS ONE 2016; 11: e0162217.




How to Cite

Yanish, Y., Zaletok, S., Vityuk, N., & Mukha, Y. (2023). Application of gold and silver nanoparticles for selective assay of spermine in mixture with spermidine. Experimental Oncology, 43(1), 77–81.



Methods and techniques

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