Drag reducing polymers attenuate adverse effects of ischemia-reperfusion upon resection of liver metastases modeled by MC38 mouse colon adenocarcinomama
DOI:
https://doi.org/10.32471/exp-oncology.2312-8852.vol-42-no-1.14095Keywords:
drag reducing polymers, ischemia/reperfusion, liver metastases, metastatic colorectal cancerAbstract
Summary. Background: The resection of metastases within healthy parenchyma improves significantly the long-term outcome in metastatic colorectal cancer. Until now, the resection technique involves Pringle maneuver, which allows reducing blood loss during transsection of liver parenchyma. However, the classical Pringle maneuver has restrictions due to ischemia/reperfusion (I/R) effect, in particular increasing risk of tumor recurrence after liver surgery. Aim: To study the pathological impact of surgical intervention and I/R effect on healthy liver tissue in the experimental setting by evaluating the markers of redox-homeostasis and oxidatively induced mutagenesis, and also to assess the current possibilities of their correction by application of drag-reducing polymers (DRPs). Materials and Methods: MC38 mouse colon adenocarcinoma cells were transplanted intrahepatically to C57Bl/6 mice. The influence of warm ischemia on metastatic potential of MC38 cells, the speed of superoxide radicals (SR) generation and 8-hydroxydeoxyguanosine content were studied. Results: In case of modeled liver metastases, the surgery initiates an increase in the rate of SR generation into the remaining liver tissue and, consequently, provokes its ischemic injury. The application of DRPs protects liver tissue under I/R conditions. Conclusions: The warm I/R can promotes metastatic lesions in the healthy part of the organ with underlying increase in the rate of SR generation and oxidatively induced damage of guanine in DNA. The hemorheological effects of DRPs ensure increase of microcirculatory perfusion and oxygenation of liver tissues with the reduction of the rate of SR generation and decrease of 8-hydroxydeoxyguanosine as a marker of oxidatively induced mutations in DNA of hepatocytes. The intraperitoneal administration of nanomolar doses of DRPs prevents the activation of the growth of dormant metastatic MC38 cells in the liver. Further experimental and clinical study of these substances will allow reducing the risks of activation of uncontrolled tumor growth in the liver due to the pathological effect of post-operative I/R.
References
Hester CA, El Mokdad A, Mansour JC, et al. Current pattern of use and impact of Pringle maneuver in liver resections in the United States. J Surg Res 2019; 239: 253–60.
Hamaguchi Y, Mori A, Fujimoto Y, et al. Longer warm ischemia can accelerate tumor growth through the induction of HIF-1α and the IL-6-JAK-STAT3 signaling pathway in a rat hepatocellular carcinoma model. J Hepatobiliary Pancreat Sci 2016; 23: 771–9.
Tohme S, Yazdani HO, Al-Khafaji AB, et al. Neutrophil extracellular traps promote the development and progression of liver metastases after surgical stress. Cancer Res 2016; 76: 1367–80.
Margonis GA, Sasaki K, Andreatos N, et al. Increased kinetic growth rate during late phase liver regeneration impacts the risk of tumor recurrence after colorectal liver metastases resection. HPB (Oxford) 2017; 19: 808–17.
Matsumoto T, Hasegawa S, Hida K, et al. Role of repeat resection in patients with metastatic colorectal cancer: a multicenter retrospective study. Dis Colon Rectum 2019; 62: 561–7.
Yamashita S, Venkatesan AM, Mizuno T. et al. Remnant liver ischemia as a prognostic factor for cancer-specific survival after resection of colorectal liver metastases. JAMA Surg 2017; 152: e172986. doi: 10.1001/jamasurg.2017.2986.
Chalissery J, Jalal D, Al-Natour Z, Hassan AH. Repair of oxidative DNA damage in Saccharomyces cerevisiae. DNA Repair (Amst) 2017; 51: 2–13.
Van Houten B, Santa-Gonzalez GA, Camargo M. DNA repair after oxidative stress: current challenges. Curr Opin Toxicol 2017; 7: 9–16.
Serracino-Inglott F, Habib NA, Mathie RT. Hepatic ischemia-reperfusion injury. Am J Surg 2001; 181: 160–6.
Tohme S, Kameneva MV, Yazdani HO, et al. Drag reducing polymers decrease hepatic injury and metastases after liver ischemia-reperfusion. Oncotarget 2017; 8: 59854–66.
Toms BA. Some observations on the flow of linear polymer solutions through straight tubes at large Reynolds numbers. Proc 1st Int Congress on Rheology 1948: 135–41.
Kameneva MV. Microrheological effects of drag-reducing polymers in vitro and in vivo. Int J Engineer Sci 2012; 59: 168–83.
Bragin DE, Kameneva MV, Bragina OA, et al. Rheological effects of drag-reducing polymers improve cerebral blood flow and oxygenation after traumatic brain injury in rats. J Cereb Blood Flow Metab 2017; 37: 762–75.
Zhao R, Marhefka J, Antaki JF, Kameneva MV. Drag-reducing polymers diminish near-wall concentration of platelets in microchannel blood flow. Biorheology 2010; 47: 193–203.
Marascalco PJ, Blair HC, Nieponice A, et al. Intravenous injections of soluble dragreducing polymers reduce foreign body reaction to implants. ASAIO J 2009; 55: 503–8.
Burlaka A, Rudiuk T, Burlaka A, Kolesnik O. 8-hydroxy-deoxyguanosine as an independent prognostic factor in patients with metastatic colorectal cancer. Arch Balk Med Union 2019; 54: 445–54.
Motta MVL, de Castro EVR, Muri EJB, et al. Study of the mechanical degradation mechanism of guar gum in turbulent flow by FTIR. Int J Biol Macromol 2019; 121: 23–8.
Burlaka AA, Vovk AV, Burlaka AP, Kolesnik OO. DNA oxidation in patients with metastastic colorectal cancer: clinical significance of 8-hydroxy-deoxyguanosine as an independent prognostic factor. Exp Oncol 2019; 41: 26–31.
Iwasaki J, Afify M, Bleilevens C, et al. The impact of a nitric oxide synthase inhibitor (L-NAME) on ischemia–reperfusion injury of cholestatic livers by Pringle maneuver and liver resection after bile duct ligation in rats. Int J Mol Sci 2019; 20: pii: E2114. doi: 10.3390/ijms20092114.
Fedosov DA, Caswell B, Popel AS, Karniadakis GE. Blood flow and cell-free layer in microvessels. Microcirculation 2010; 17: 615–28.
Tohme S, Yazdani HO, Al-Khafaji AB, et al. Neutrophil extracellular traps promote the development and progression of liver metastases after surgical stress. Cancer Res 2016; 76: 1367–80.
Labelle M, Begum S, Hynes RO. Platelets guide the formation of early metastatic niches. Proc Natl Acad Sci USA 2014; 111: E3053–61.
Huang H, Tohme S, Al-Khafaji AB, et al. Damage-associated molecular pattern-activated neutrophil extracellular trap exacerbates sterile inflammatory liver injury. Hepatology 2015; 62: 600–14.
Pacella JJ, Kameneva MV, Csikari M, et al. A novel hydrodynamic approach to the treatment of coronary artery disease. Eur Heart J 2006; 27: 2362–9.
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