Oxidative and mutagenic effects of low intensity GSM 1800 MHz microwave radiation
Keywords:
carcinogenesis, embryo, microwaves, mutagenic effects, oxidative effects, radiofrequency, reactive oxygen speciesAbstract
Summary. Aim: Despite a significant number of epidemiological studies on potential carcinogenicity of microwave radiation (MWR) from wireless devices and a bulk of experimental studies on oxidative and mutagenic effects of low intensity MWR, the discussion on potential carcinogenicity of low intensity MWR is going on. This study aims to assess oxidative and mutagenic effects of low intensity MWR from a typical commercial model of a modern smartphone. Materials and Methods: The model of developing quail embryos has been used for the assessment of oxidative and mutagenic effects of Global System for Mobile communication (GSM) 1800 MHz MWR from a commercial model of smartphone. The embryos were exposed in ovo to 0.32 µW/cm2, discontinuously — 48 s — On, 12 s — Off, during 5 days before and 14 days through the incubation period. Results: The exposure of quail embryos before and during the incubation period to low intensity GSM 1800 MHz has resulted in expressive statistically significant oxidative effects in embryonic cells, including a 2-fold increase in superoxide generation rate and 85% increase in nitrogen oxide generation rate, damages of DNA integrity and oxidative damages of DNA (up to twice increased levels of 8-oxo-dG in cells of 1-day old chicks from the exposed embryos). Finally, the exposure resulted in a significant, almost twice, increase of embryo mortality. Conclusion: The exposure of model biological system to low intensity GSM 1800 MHz MWR resulted in significant oxidative and mutagenic effects in exposed cells, and thus should be recognized as a significant risk factor for living cells.
References
Baan R, Grosse Y, Lauby-Secretan B, et al. Carcinogenicity of radiofrequency electromagnetic fields. Lancet Oncol 2011; 12: 624–6.
Hardell L. World Health Organization, radiofrequency radiation and health-a hard nut to crack. Int J Oncol 2017; 51: 405–13.
Yakymenko I, Tsybulin O, Sidorik E, et al. Oxidative mechanisms of biological activity of low-intensity radiofrequency radiation. Electromagn Biol Med 2016; 35: 186–202.
Ruediger HW. Genotoxic effects of radiofrequency electromagnetic fields. Pathophysiology 2009; 16: 89–102.
Chekhun V, Yakymenko I, Sidorik E, Tsybulin O. Current state of international and national public safety limits for radiofrequency radiation. Sci J Minist Health Ukr 2014: 57–64 (in Ukrainian).
Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175: 184–91.
Hartmann A, Agurell E, Beevers C, et al. Recommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop. Mutagenesis 2003; 18: 45–51.
Tsybulin O, Sidorik E, Brieieva O, et al. GSM 900 MHz cellular phone radiation can either stimulate or depress early embryogenesis in Japanese quails depending on the duration of exposure. Int J Radiat Biol 2013; 89: 756–63.
Buettner G, Mason R. Spin-trapping methods for detecting superoxide and hydroxyl free radicals in vitro and in vivo. In: Critical Reviews of Oxidative Stress And Aging: Advances In Basic Science, Diagnostics And Intervention. Cutler R, Rodriguez R, eds.New Jersey, London, Singapore, Hong Kong: World Scientific, 2003: 27–38.
Olishevsky S, Burlaka A, Sidorik E, et al. Modulation of ros/no production by murine peritoneal macrophages in response to bacterial CpG DNA stimulation. Exp Oncol 2006; 28: 114–20.
Lai CS, Komarov AM. Spin trapping of nitric oxide produced in vivo in septic-shock mice. FEBS Letters 1994; 345: 120–4.
Shigenaga MK, Gimeno CJ, Ames BN. Urinary 8-hydroxy-2′-deoxyguanosine as a biological marker of in vivo oxidative DNA damage. Proc Natl Acad Sci USA 1989; 86: 9697–701.
Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1990; 186: 421–31.
Tsybulin O, Sidorik E, Kyrylenko S, Henshel D, Yakymenko I. GSM 900 MHz microwave radiation affects embryo development of Japanese quails. Electromagn Biol Med 2012; 31: 75–86.
Chavary S, Chaba I, Sekuy I. Role of superoxide dismutase in cellular oxidative processes and method of assessment of its biological activity. Lab Delo 1985; 11: 678–81 (in Russian).
Koroliuk M. Method of catalase activity assessment. Lab Delo 1988; 1: 16–9 (in Russian).
Ten E. Express test on ceruloplasmin activity in blood serum. Lab Delo 1981: 334–5 (in Russian).
De Iuliis GN, Newey RJ, King BV, Aitken RJ. Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro. PLoS One 2009; 4: e6446.
Friedman J, Kraus S, Hauptman Y, et al. Mechanism of short-term ERK activation by electromagnetic fields at mobile phone frequencies. Biochem J 2007; 405: 559–68.
Inoue M, Sato Eisuke F, Nishikawa M, et al. Mitochondrial generation of reactive oxygen species and its role in aerobic life. Curr Med Chem 2003; 10: 2495–505.
Liu Y, Fiskum G, Schubert D. Generation of reactive oxygen species by the mitochondrial electron transport chain. J Neurochem 2002; 80: 780–7.
Guzy RD, Schumacker PT. Oxygen sensing by mitochondria at complex III: the paradox of increased reactive oxygen species during hypoxia. Exp Physiol 2006; 91: 807–19.
Brown GC. Nitric oxide and mitochondria. Front Biosci 2007; 12: 1024–3.
Burlaka A, Tsybulin O, Sidorik E, et al. Overproduction of free radical species in embryonic cells exposed to low intensity radiofrequency radiation. Exp Oncol 2013; 35: 219–25.
Tsybulin O, Sidorik E, Yakymenko I. Oxidative effect of low intensity microwave radiation in the model of developing quail embryos. Oxid Antioxid Med Sci 2017; 6: 9–13.
Agarwal A, Desai NR, Makker K, et al. Effects of radiofrequency electromagnetic waves (RF-EMW) from cellular phones on human ejaculated semen: an in vitro pilot study. Fertil Steril 2009; 92: 1318–25.
Furtado-Filho OV, Borba JB, Dallegrave A, et al. Effect of 950 MHz UHF electromagnetic radiation on biomarkers of oxidative damage, metabolism of UFA and antioxidants in the livers of young rats of different ages. Int J Radiat Biol 2014; 90: 159–68.
Gürler HS, Bilgici B, Akar AK, et al. Increased DNA oxidation (8-OHdG) and protein oxidation (AOPP) by low level electromagnetic field (2.45 GHz) in rat brain and protective effect of garlic. Int J Radiat Biol 2014; 90: 1–5.
Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39: 44–84.
Valko M, Rhodes CJ, Moncol J, et al. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 2006; 160: 1–40.
Cooke MS, Evans MD, Dizdaroglu M, Lunec J. Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J 2003; 17: 1195–214.
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