STAGE OF DIFFERENTIATION, PROLIFERATIVE INDEX OF TUMOR CELLS AND CYTOTOXIC ACTIVITY OF PERIPHERAL BLOOD LYMPHOCYTES IN COLORECTAL CANCER PATIENTS

Paholyuk Tatyana D., Zacharzeva Lubov M., Koshel' Konstantin V., Oliynichenko Gennadiy P., Berezhnaya Ninel M.

Aim: To evaluate prognostic value of activity of cytotoxic lymphocytes of peripheral blood (CTL) of patients with colorectal cancer (CRC). Methods: 2-year survival of 30 patients with colorectal cancer stage II (T_2–3N_0–1M₀G_1–3) was investigated in relation with clinical data, cytotoxic activity of peripheral blood lymphoсytes (by МТТ-test) and immunohistochemical peculiarities of tumor cells using anti-Ki-67-MABs. Results: It was revealed that some factors had prognostic significance, namely regional lymph nodes involvement (r = –0.69), proliferative activity of tumor cells (Ki-67-positivity) (r = –0.67), restoration of CTL function after operation (r = 0.46), adjuvant chemotherapy (r = 0.41) and differentiation stage of tumor (r = 0.40). Negative correlation between CTL activity before operation and tumor proliferative activity also exists (r = –0.35). Conclusion: the data point to the involvement of natural killer cells in the control of tumor growth.