EVALUATION OF CISPLATIN-DNA CROSSLINKS FORMATION WITH UV-C APPLICATION BY THE ALKALINE COMET?ASSAY
The aim of the present study was to investigate DNA damage induced by widely applied anticancer preparation cisplatin (cis-DDP) at doses comparable with therapeutic ones in leukocytes of healthy donors. Methods: DNA damage and repair was estimated by the single cell gel electrophoresis or Comet-assay. For estimation of cis-DDP-induced DNA crosslinks the combined treatment by cis-DDP with the DNA damaging agent UV-C was used. The results obtained indicate that cis-DDP is forming crosslinks with DNA in human leukocytes and significantly reduce UV-C-induced DNA migration. Conclusion: The Comet-assay with UV-C application is a useful tool to detect cis-DDP- induced DNA crosslinks. Data on the cis-DDP-induced DNA damage in vitro may be important for their extrapolation on in vivo level.
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