ROLE OF POLYAMINES IN THE FUNCTION OF NUCLEAR TRANSCRIPTION FACTOR NF-kB IN BREAST CANCER CELLS
The aim of the work was the investigation of the influence of polyamines on formation of nuclear transcription factor kappa B (NF-kB) complex with specific DNA-sequences nuclear response elements (NRE), and the study of content of p50 and p65 (NF-kB subunits) in breast tumor cells under polyamines metabolism modulation. Methods: The model experiments in vitro were carried out on cultured MCF-7 cells, in vivo experiments — on experimental Ca755 and Walker-256 tumors using surface plasmon resonance (SPR) and Western-blotting analysis. Results: The data have demonstrated that polyamines falicitated p50 — NRE binding. Under conditions of polyamine depletion, an increase of content of both p50 and p65 subunits of NF-kB in the nucleus has been registered (especially that of p50 subunits). Upon addition of a-DFMO + putrescine to the culture medium of MCF-7 cells, the decrease of the levels of p50 and p65 proteins in the nuclei has been observed. Conclusion: Polyamines influence the activity of NF-kB transcription factor in breast tumor cells.
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