Shlyakhovenko V.O.*, Pyaskovskaya O.N., Dasyukevich O.I., Garmanchouk L.V., Yanish Yu.V.

Aim: To evaluate the influence of new antitumor preparation BC1 produced on the base of Aconitum species on viability and electrokinetic properties of endothelial cells for estimation of mechanisms of its antitumor and antimetastatic activity. Materials and Methods: Cytotoxic/cytostatic action of BC1 toward murine aorta endothelial cells (МАЕС) and tumor LLC/R9 cells was studied using МТТ-test. Apoptotic rate of MAEC was performed by flow cytometry. Electrokinetic properties of МАЕС were determined by linear rate of their migration in electric field with the voltage of 20 V/cm. Results: After 24 h of incubation with BC1, IC50 for actively proliferating МАЕС was 0.95 ± 0.06 µg/ml and was 9-fold and 14-fold lower (p < 0.05) than that index for confluent endotheliocytes and LLC/R9 cells respectively. The ability of BC1 to alter electrokinetic characteristics of MAEC and to induce apoptosis has been demonstrated. At the concentration of IC50/10, ВС1 caused 2-fold decrease of z-potential and surface density of charge of МАЕС compared to the control (p < 0.05) whilst at the concentration of IC50/20 – inversion of surface charge of the majority (80%) of the cells in association with BC1-induced apoptosis. Conclusion: High sensitivity of actively proliferating MAEC to the action of BC1 was revealed as well as the ability of that preparation to cause apoptosis and inversion of surface charge of endothelial cells.

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