THE SENSITIVITY OF CHEMIORESISTANT HUMAN TUMOR EXPLANTS TO LYSIS BY ACTIVATED ANDNONACTIVATED AUTOLOGICAL LYMPHOCYTES: A PILOT STUDY

Berezhnaya N.M., Vinnichuk U.D., Konovalenko V.F.¹, Vorobjova L.I.², Belova O.B., Proskurnya L.A.³

• ¹R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine
• ²Institute of Oncology, AMS of Ukraine, Kyiv 03022, Ukraine
• ³National Cancer Institute, Kyiv, 03022, Ukraine

Summary. Aim: The comparative study of antitumor action of peripheral blood lymphocytes (PBL) and lymphokin-activated killer cells (LAK) on autologous cell of chemoresistant and chemosensitive human soft tissue sarcomas and tumors of epithelial origin. Materials and Methods: Tumor explants (15 samples of soft tissue sarcomas, 10 samples of cervical and ovarian carcinomas) were cultivated with autologous lymphocytes in double diffusion chambers. Evaluation of the results was done on the basis of morphological criteria of explants growth. Results: The results have shown that in the patients with the resistant soft tissue sarcomas and carcinomas LAK possess more pronounced antitumor action, than non-activated lymphocytes, whilst PBL possess higher antitumor action on sensitive epithelial tumors than that on soft tissue sarcoma. Conclusion: LAK possess antitumor action toward chemoresistant tumors of different origin and localization.