EFFECT OF LANDOMYCIN E ON EXPRESSION OF mRNA CODING FOR TRANSFORMING GROWTH FACTOR b LIGANDS AND SPECIFIC RECEPTORS IN MCF-7 CELLS

Panchuk R., Korynevska A., Stoika R.

The aim of the present study was to investigate regulation of mRNA, coding for TGFb ligands and specific receptors, by novel antitumor antibiotic landomycin E (LE) in human breast adenocarcinoma cells of MCF-7 line, sensitive and resistant to anti-cancer agent doxorubicin. Methods: Semi-quantitative analysis of mRNA expression was estimated using RT-PCR and DNA gel-electrophoresis methods. For comparison, another anti-cancer drug doxorubicin (adriamycin) was utilized. The results obtained indicate that LE induces up-regulation of mRNA coding for TGFb receptors type I and type II in MCF-7 cells, as well as in their doxorubicin-resistant sub-line. Conclusion: Anti-tumor action of LE may be mediated by TGFb, and a signaling pathway of this cytokine is not affected by the development of tumor cell resistance to doxorubicin.