AN IN VITRO STUDY OF CYTOTOXIC EFFECTS OF GOSSYPOL ON HUMAN EPIDERMOID LARYNX CARCINOMA CELL LINE (HEp-2)

Konac Ece, Ekmekci Abdullah, Yurtcu Erkan, Ergun Mehmet Ali

Aim: The study was aimed on an evaluation of the effect of gossypol at concentration of 25–150 mM on the level of apoptosis in the human epidermoid larynx carcinoma cell line, HEp-2, in vitro. Methods: To determine the apoptotic effects of gossypol on growth of human epidermoid larynx carcinoma cell lines (HEp-2 cells), morphological evaluation of the cells and DNA fragmentation analysis were used. Results: 6 h incubation of HEp-2 cells with gossypol at concentrations of 50 mM and 75 mM resulted in the increase of apoptosis rate by 26 and 23%, respectively. Conclusion: This preliminary study may constitute a base for in vivo studies of anticancer properties of gossypol.