THE EFFECT OF HYPOXIA ON PHOTOCYTOTOXICITY OF TICS TRICARBOCYANINE DYE IN VITRO

Istomin Y.P., Alexandrova E.N., Zhavrid E.A., Voropay E.S., Samtsov M.P., Kaplevsky K.N., Lugovsky A.P., Lugovsky A.А.

Aim: To evaluate the effect of cell oxygenation on photocytotoxicity of a novel tricarbocyanine indolenine dye covalently bound to glucose (TICS). Methods: HeLa cells were incubated with 5 µM TICS, 2 h later irradiated by laser at 740 nm with a light dose of 10 J/cm², delivered at a power density of 10, 20, 25 or 30 mW/cm², in air or in argon atmosphere, and then scored for viability. Results: The photocytotoxicity of TICS increased dramatically as the power density was reduced. Under hypoxia TICS-photosensitized cell death was determined but its value was lowered, compared to photoirradiation in the air. Conclusion: Photosensitizing effect of TICS is only partially dependent on the oxygenation of tumor cells.