HIGH FORMATION OF SUPEROXIDE ANION AND NITRIC OXIDE, AND MATRIX METALLOPROTEINASES ACTIVITY IN VASCULAR WALL OF RECTAL CARCINOMA VESSELS

Burlaka A.P.*¹, Sidorik E.P.², Ganusevich I.I.¹, Lestchenko Yu.M.³, Burlaka A.P.¹, Osinsky S.P.¹

• ¹R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine
• ²R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kyiv 03022, Ukraine
• ³L.P. Shupik Kyiv Medical Academy of Postgraduate Education, MH of Ukraine,Kyiv, Ukraine

Summary. Aim: To study the relationship between the level of generation of reactive oxygen species (ROS) and nitric oxide (NO) and activity of matrix metalloproteinases (MMPs) MMP-2 and MMP-9 in the vessels isolated from rectal tumors and Arteria rectalis superior. Methods: EPR at the room temperature and 77 °K, Spin Traps technology and zymography in polyacrylamide gels were applied. Results: In the vessels isolated from rectal tumors and Arteria rectalis superior high levels of ROS, NO^· and formation of complexes of NO^· with FeS-proteins at the sites of electron-transporting chain of mitochondria have been detected. High activities of MMP-2 and MMP-9 in vascular wall were also observed. The direct positive correlation between the rate of NO^· generation and formation of complexes of NO^·· with FeS-proteins as well as between ROS and NO formation and MMPs activities have been revealed. Conclusion: Altered oxidative equilibrium in mitochondria of cells in vascular wall promotes formation of cell hypoxia and its autocatalytic potentiation accompanied with activation of MMPs.