Characteristic of HLA-genotype in children with all and AML

Takun K., Savitskaya T.V., Aleinikova O.V.

K. Takun, T. Savitskaya, O. Aleinikova

National Research Center for Pediatric Oncology, Hematology and Immunology, Minsk, Belarus

IntroductionThe immunogenetic study has disclosed the correlation between HLA alleles and diseases. Investigated alleles could be used as genetic markers in the diagnosis of malignant diseases. However, the results of an immunogenetic research obtained in any population can not be automatically transferred to other ethnic groups. The HLA-distribution specificity analysis in acute leukemia patients is of primary importance. This information can help practitioners to reveal individuals with a high probability of malignant diseases. Aim: The HLA-genotype analysis in children suffering from acute leukemia and in children with non-malignant diseases. Marker specificy detection of high and low risk of leukemia progression. Methods: 25 children with acute lymphoblastic leukemia (ALL), 16 children with acute myeloblastic leukemia (AML) and 16 children with non-malignant diseases were included in the study. HLA-genotyping of leucocytes was performed by PCR-SSO (sequence specific oligonucleotide) and PCR-SSP (sequence specific primers) with reagent kits (Invitrogen, USA). The significance of discrepancy between groups was assessed by using chi-squared test. Results: The distribution pattern of HLA-alleles in two subgroups of patients with acute leukemia has been analyzed. The most frequent alleles in ALL patients were A*02 (15 patients, 34%), A*24 (9 patients, 18%), B*35 (9 patients, 18%), B*07 (6 patients, 12%), C*07 (9 patients, 20%), C*04 (6 patients, 12%), DRB1*01 (9 patients, 18%), DRB1*04 (8 patients, 16%), DQB1*05 (11 patients, 50%), DQB1*03 (15 patients, 36%). The most frequent alleles in AML patients were A*02 (8 patients, 34%), A*24 (4 patients, 16%), B*35 (5 patients, 16%), B*51 (3 patients, 13%), C*07 (6 patients, 22%), C*06 (6 patients, 22%), DRB1*07 (6 patients, 22%), DRB1*11 (5 patients, 19%), DQB1*03 (7 patients, 30%), DQB1*02 (7 patients, 27%). In patients with non-malignant diseases HLA-alleles distribution was the following: A*02 (8 patients, 31%), A*03 (5 patients, 19%), B*44 (8 patients, 28%), B*08 (4 patients, 13%), C*07 (10 patients, 31%), C*04 (7 patients, 25%), DRB1*03 (5 patients, 19%), DRB1*15 (6 patients, 19%), DQB1*02 (9 patients, 31%), DQB1*06 (9 patients, 28%). We displayed that differences between groups of ALL patients and patients with non-malignant diseases on НLА-DRB1*03 are statistically significant (p = 0.04). Conclusion: Analysis of HLA-DRB*03 locus has revealed the significant difference in ALL patients when compared with the group of children with non-malignant diseases. Obtained results are of certain interest and require further investigation.

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