Influence of two Pt(IV) complexes on viability, apoptosis and cell cycle of B16 mouse melanoma tumors
Several platinum(IV) complexes are showing considerable promise in initial trials, producing reactive intermediates that then interact with DNA. Aim: To perform in vitro study of two new platinum(IV) complexes cytotoxic effect on B16 mouse melanoma cells. Methods: PtCl4(dbtp)2 and PtCl2(6mp)2 complexes were prepared. PtCl4(dbtp)2 was created as modification of PtCl4(dmtp) test previously. Apoptosis and necrosis were examined using flow cytometry, upon Annexin V/PI staining. Results: LC10, LC50 and LC90 parameters established for PtCl4(dbtp)2 were as following: 2.6, 17.0, 58.0 μmol/L. However LC10 and LC50 established for PtCl2(6mp)2 were 1.2 and 14.0 μmol/l respectively. The both complexes induced apoptosis. PtCl2(6mp)2 induced cell cycle arrest in G0/G1, while PtCl4(dbtp)2 — in S-phase. Conclusions: PtCl4(dbtp)2 appeared to be more cytotoxic against B16 cells than PtCl2(6mp)2. Apoptosis was the main mechanism of cell loss in cultures incubated with both tested complexes.
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