NEW APPROACHES FOR THYROCYTE CULTIVATION IN VITRO WITH RETENTION OF THEIR FOLLICULAR ORGANIZATION

Khoruzhenko A.I.

In the presented work the human thyrocyte cultures obtained from nodular goiter tissue with and without retention of follicular structure were compared in regard to the manifestation of thyrocyte functional activity in vitro. As initial material for primary cultures, isolated undamaged follicles instead of single cell suspension were used. The suspension of follicles was derived from the surgical specimens of human thyroid obtained from patients with Nodular goiter. The quantity of follicles isolated from neoplastic tissue was significantly higher than from surrounding normal tissue. Subsequently the monolayer culture, multicellular spheroids, and the floating aggregates of follicles were studied. Essentially all the cells in obtained cultures were identified as epithelial. The presence of intracellular thyroglobulin, which was detected by immunoperoxidase staining, was used as an index of functional activity of cultivated thyrocytes under different conditions. In contrast to the monolayer culture and multicellular spheroids, the thyrocytes from aggregates of follicles demonstrated ubiquitous positive reaction for thyroglobulin. If summarize, culture obtained from this benign thyroid tumor, with retention of follicular organization in vitro, offers an useful model for investigation of thyrocyte growth and differentiation.

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