THE SYNCHRONOUS DETECTION OF HPV, H-ras AND p53 IN CERVICAL CARCINOMA TISSUES AND DISTRIBUTION OF HPV INFECTION BETWEEN THE HIGH INCIDENCE AREA AND THE COMMON AREA
In the present study, the synchronous detection of HPV and HPV16-E7, mutations of H-ras and p53 genes and comparison of HPV infection rate in population of high incidence and common areas was performed using PCR and SSCP-PCR techniques. HPV infection was detected in 85% (34/40) of cervical cancer specimens and HPV16-E7 gene was found in 61,7% (21/34) of the HPV infected tissues. H-ras mutation was detected in 23.5% (8/34) of the HPV-positive tissues and in 38% (8/21) of HPV16-E7 positive tissues. p53 mutation was revealed in 2.9% of HPV-positive tissues. In addition, HPV was present in 87,5% and 85% of patients from high incidence and common areas, respectively. HPV infection was detected in 32.7% patients with cervicitis from high incidence area and only in 6.7% patients from common area (p < 0.05). In conclusion, HPV16-E7 gene is closely associated with cervical cancer. The mutations of H-ras and HPV16-E7 genes may cooperate in cervical cancer progression. The mutations of p53 gene (exon 5–6) are not universal in cervical cancer. The rate of HPV infection in patients with cervicitis is significantly higher in high incidence area that in common area.
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