THE ROLE OF EXPRESSION OF THE COMPONENTS OF PROTEOME IN THE FORMATION OF MOLECULAR PROFILE OF HUMAN OVARIAN CARCINOMA А2780 CELLS SENSITIVE AND RESISTANT TO CISPLATIN
Aim: To study the expression of proteins that characterize drug resistance, proliferation and apoptosis of human ovarian carcinoma cells. Methods: The study was carried out on human ovarian carcinoma А2780 cells and on the A2780/DDP8 subline resistant to cisplatin. Expression of the surface and intracellular antigens (р53, Bcl-2, CD95, antigen of proliferating cells, metallothioneins, drug resistance proteins (P-glycoprotein (P-gp), glutathione-S-transferase), molecules of adhesion (Е-cadherin, a- and b-catenins) was studied by immunocytochemical method. Results: It has been shown that the formation оf the resistance to cisplatin in A2780/DDP8 cells is accompanied by the increase of expression of glutathione-S-transferase and Bcl-2, by the decrease of expression of CD95-antigene and proliferation potential of the cells, by appearance of EGF receptors and elevation of expression level of E-cadherin, a- and b-catenins, proving the enhancement of adhesive properties of tumor cells. Conclusion: Antiapoptotic program seems to be the leading mechanism of the development of the resistance to cisplatin in A2780/DDP8 cells and is realized via high expression of Bcl-2. During the development of drug resistance of A2780/DDP cells, the program of glutathione detoxification is functionally replacing the decrease of the content of metallothioneins.
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