RESPONSE OF THE THYMUS AND SPLEEN OF CBA MICE WITH SARCOMA 37 ON INTRAVENOUS AND SUBCUTANEOUS ADMINISTRATION OF SYNGENEIC DENDRITIC CELLS

Grinevich Yu.A., Khranovskaya N.N., Bendyug G.D.

Objective: To compare the effect of intravenous (i/v) and subcutaneous (s/c) infusion of syngeneic dendritic cells (DC) loaded or unloaded with lyzate of tumor cells (LTC) on apoptosis and proliferation of thymic and splenic cells in CBA mice with sarcoma 37 (S 37). Methods: Experiments were carried out on CBA mice bearing transplanted ascitic form of S 37. DC loaded and unloaded with LTC were infused i/v and s/c (2x105 per animal) every 4 days (3 injections in total). Apoptosis and proliferation of thymic and splenic cells were evaluated using flow cytofluorimetry. Results: It has been found that tumor development is accompanied by altered apoptosis and proliferation level in thymus and spleen. I/v infusion of syngeneic DC loaded and unloaded with LTC rendered the normalizing effect on the state of thymus in spleen in tumor-bearing animals. In the case of s/c infusion such effect of DC is less expressed. The use of DC, loaded with LTC irrespectively of the way of administration renders more durable effect on the functional state of these cells than the use of unloaded DC. Conclusion: Injection of syngeneic DC both loaded or unloaded with LTC renders a normalizing effect on the state of organs of immune system in tumor-bearing CBA mice.

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