DETECTION OF p53 DELETIONS USING FISH TECHNIQUE IN THE HL-60, DAUDI, RAJI AND K-562 CELL LINES

Konac E., Ergun M.A., Sahin F.I., Ekmekci A.

The work is aimed on the analysis of chromosome 17 centromere and p53 gene-specific signals within interphase and metaphase, determination of p53 deletion ratios and the detection of the cell-cycle progress. The loss of heterozygosity (LOH) has been investigated using FISH method in 4 cell lines (HL-60, Daudi, Raji and K-562) and peripheral blood samples from 3 Burkitt’s lymphoma patients and 2 healthy donors. It is concluded that FISH method may be used in aneuploidy as well as in detection of p53 deletions and mutations.