THE SERUM OF CARCINOMA PATIENTS POWERFULLY ACTIVATES CARBONIC ANHYDRASE II

Puscas I., Puscas C., Coltau M., Baican M., Domuta G.

The effects of the serum of cancer patients on carbonic anhydrase (CA) I and II activity have been studied. In total 2134 samples of the serum from patients with different types of cancer as well as non-cancer patients and healthy persons have been analyzed. CA activity was assayed by a stopped-flow method with a rapid kinetic device HI-TECH SF51MX. All the sera of cancer patients under study, no matter of the type of cancer, turned out to be able to activate CA II. The activating effect was registered at a 1 : 100 dilution and increased progressively up to 1 : 10 dilution reaching the peak of the activation. The results suggest the presence of some endogenous CA II activators in the serum of cancer patients.