COMPARATIVE STUDY OF NONHISTONE CHROMOSOMAL pp23 PROTEIN IN NORMAL AND TUMOR RAT CELLS

Grandilevskaya A.B., Gruzdeva I.G., Ivanov V.A.

A specific immune serum was raised against the tumor-associated pp23 protein from Zajdela hepatoma cells. With the aid of this immune serum and the immune serum against the same protein from intact rat kidney cells, using immunoblotting and affinity chromatography, an immunological similarity of pp23 proteins from rat kidney and Zajdela hepatoma was shown. pp23 preparations isolated from cells of kidney and Zajdela hepatoma were shown to be equally effective in induction of membrane tumor-associated protein expression on the surface of intact rat hepatocytes. Based on these data, it may be concluded that pp23 isolated from rat kidney cells and Zajdela hepatoma cells are practically identical while possible differences at the molecular level do not affect immunological and functional properties of these proteins.