EFFECTS OF CONDITIONED MEDIA OF PRIMARY HUMAN GASTRIC CARCINOMAS ON VASCULAR ENDOTHELIUM CELL ADHESIVENESS IN VITRO

Hipp I.G., Volodko N.A., Barylka V.A., Piddubnyak V.A., Bilynsky B.T.

We have investigated the effects of the conditioned medium of primary cultures of gastric cancer on the human umbilical vascular endothelium cell culture (HUVEC) and analyzed the correlations of these effects with pathomorphological features of the tumors as well as TNF and TGF-b concentrations in supernatants. The adhesion index (AI) of L929 cells to the HUVEC after stimulation by supernatants of the poorlydifferentiated gastric carcinomas was 1.67 ± 0.18, and supernatants of well differentiated carcinomas — 1.2 ± 0.04 (P < 0.05). AI of L929 cells to the HUVEC after his stimulation by supernatants of Bormann type I–II carcinomas was 1.2 ± 0.04, by supernatants of Bormann type III carcinomas — 1.6 ± 0.18 and by supernatants of Bormann type IV carcinomas — 1.5 ± 0.06 (P < 0.001). The conditioned medium of the gastric cancer cell culture enhanced the adhesive properties of the vascular endothelium. The poorly differentiated tumors stimulated HUVEC more than well differentiated ones. We have not found any correlation between TNF and TGF-b concentration in supernatants and AI.

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