REGULATION OF EXPRESSION OF THE COMPONENTS OF PLASMINOGEN ACTIVATION SYSTEM IN THE LEUKEMIC CELLS

Antonyak H., Babych N., Solohub L., Snitynski V., Binder B.

In present research the abilities of leukemic cell lines K562, U937 and THP-1 to modulate expression of the components of plasminogen activation system under the influences of tumor promoter, phorbol myristate acetate (PMA), and antiproliferative agents, tumor necrosis factor a (TNF-a) and interferon-a (IFN-a) were studied. PMA was found to cause the significant increase in urokinase receptor (uPAR) and urokinase (uPA) molecules expression in both permeabilized and non-permeabilized cells of the investigated lines. TNF-a stimulated expression of uPA and plasminogen activator inhibitor type-1 (PAI-1) molecules in permeabilized THP-1 cells, and promoted the decrease in surface level of uPAR molecules in THP-1 cells. IFN-a did not influence the cell surface levels of the components of plasminogen activation system, but markedly stimulated the intracellular expression of PAI-1 in U937 and THP-1 cells. At the same time IFN-a decreased a rate of uPAR molecules expression in permeabilized THP-1 cells. The data suggest, that influence of TNF-a and IFN-a on the expression of the components of plasminogen activator system can represent a link in the mechanisms of antitumor activities of investigated cytokines and can mediate their inhibitory action on several functions of leukemic cells.

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