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INTERFERON-ALPHA ENHANCES DEVELOPMENT OF APOPTOSIS INDUCED BY VARIOUS AGENTS IN TUMOR CELLS IN VITRO
Interferon alpha (IFN) has been shown to increase in vitro cytotoxicity of anticancer agents, in particular tumor necrosis factor (TNF) and vinblastin (VBL), on tumor cell lines (U-937, K-562, HEp-2). This effect seems to be due to IFN ability to enhance apoptosis resulting in increasing number of apoptotic cells and increasing oligonucleosomal DNA fragmentation. TPA-induced differentiation of U-937 cells protects them from apoptosis induced by various agents (TNF, VBL). In contrast to U-937 cells, which require only 30 min of incubation with IFN for realization of its proapoptotic activity, increase of TFN cytotoxicity in K-562 and HEp-2 cells demands continuous presence of IFN in the medium. Such a phenomenon could provide the grounds for further analysis of IFN proapoptotic activity in vivo.
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