Vol. 31, No. 2, 2009 (June)
	Content

Expression profile of nuclear receptors upon Epstein — Barr virus induced B cell transformation

S.P. Yenamandra^{1, 2}, A. Lundin^{1, 2} V. Arulampalam^{1, 2}, M. Yurchenko³, S. Pettersson^{1, 2}, G. Klein¹, E. Kashuba^{1, 2, 3}

¹Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institute, Stockholm S17177, Sweden ²Center for Integrative Recognition in the Immune System (IRIS), Karolinska Institute, Stockholm S17177, Sweden ³R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Kiev 03022, Ukraine

Abstract. Background: Infection of human B cells with Epstein — Barr virus (EBV) induces metabolic activation, morphological transformation, cell proliferation and eventual immortalization. Aim: To identify the nuclear receptors, which are the cellular interaction partners of EBNAs, that will help to elucidate the mechanism of B cell transformation. Methods: We have compared the nuclear receptor profile in the naïve and EBV-transformed B-lymphocytes, using TaqMan LDA microfluidic card technology. Results: Out of 48 nuclear receptor, 17 showed differential expression at the mRNA level. The expression of 5 genes was elevated in EBV-transformed cells, whereas 12 genes were downregulated in lymphoblastoid cells (LCLs). 7 genes were studied at the protein level; 2 genes were up regulated (Nr2F2 and RARA) and 4 genes were down regulated (ERB, NUR77, PPARG, and VDR) in LCLs. Conclusion: The nuclear receptor profiling on EBV infected B cells showed alterations of nuclear receptors expression at both mRNA and protein levels compared with non infected peripheral blood cells. Further analysis on a possible role of each nuclear receptor in EBV induced cell transformation should be performed.

Key Words: EBV, cell transformation, nuclear receptors, expression pattern, microarrays

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