EFFECTS OF INTERFERON-ALFA-2a ON HUMAN HEPATOMA HepG2 CELLS

Ozgur O., Karti S., Sonmez M., Yilmaz M., Karti D., Ozdemir F., Ovali E.

In the present work, the effect of interferon-alfa-2a on human hepatoma HepG2 cells has been studied. HepG2 cells were cultured with different concentrations of IFN-a of for 7 days, and then apoptotic and proliferative indexes were evaluated. It has been shown that upon such incubation, hepatoma cells were arrested in G0/G1 phase; proliferative indexes were found to be decreased significantly and apoptosis rate was increased at IFN-a concentrations of 50 U/ml and 100 U/ml compared to the control (29% ± 4.5% and 27% ± 4.6% vs 44% ± 3.2%, p < 0.01; and 34% ± 9.6% and 43% ± 22% vs 0.8% ± 1.3%, p < 0.01 respectively). Colony counts per well decreased significantly at IFN-a concentrations of 100 U/ml compared to control. Clinical relevance of these finding remain to be established with further studies.

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