EVALUATION OF CISPLATIN-DNA CROSSLINKS FORMATION WITH UV-C APPLICATION BY THE ALKALINE COMET?ASSAY

Hovhannisyan Galina G., Haroutunyan Tigran S., Arutyunyan Rouben M.

The aim of the present study was to investigate DNA damage induced by widely applied anticancer preparation cisplatin (cis-DDP) at doses comparable with therapeutic ones in leukocytes of healthy donors. Methods: DNA damage and repair was estimated by the single cell gel electrophoresis or Comet-assay. For estimation of cis-DDP-induced DNA crosslinks the combined treatment by cis-DDP with the DNA damaging agent UV-C was used. The results obtained indicate that cis-DDP is forming crosslinks with DNA in human leukocytes and significantly reduce UV-C-induced DNA migration. Conclusion: The Comet-assay with UV-C application is a useful tool to detect cis-DDP- induced DNA crosslinks. Data on the cis-DDP-induced DNA damage in vitro may be important for their extrapolation on in vivo level.

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