ROLE OF POLYAMINES IN THE FUNCTION OF NUCLEAR TRANSCRIPTION FACTOR NF-kB IN BREAST CANCER CELLS

Zaletok Sophia, Alexandrova Nataliya, Berdynskykh Nina, Ignatenko Nataliya, Gogol Sergiy, Orlovsky Oleksiy, Tregubova Nadiya, Gerner Eugene, Chekhun Vasyl

The aim of the work was the investigation of the influence of polyamines on formation of nuclear transcription factor kappa B (NF-kB) complex with specific DNA-sequences nuclear response elements (NRE), and the study of content of p50 and p65 (NF-kB subunits) in breast tumor cells under polyamines metabolism modulation. Methods: The model experiments in vitro were carried out on cultured MCF-7 cells, in vivo experiments — on experimental Ca755 and Walker-256 tumors using surface plasmon resonance (SPR) and Western-blotting analysis. Results: The data have demonstrated that polyamines falicitated p50 — NRE binding. Under conditions of polyamine depletion, an increase of content of both p50 and p65 subunits of NF-kB in the nucleus has been registered (especially that of p50 subunits). Upon addition of a-DFMO + putrescine to the culture medium of MCF-7 cells, the decrease of the levels of p50 and p65 proteins in the nuclei has been observed. Conclusion: Polyamines influence the activity of NF-kB transcription factor in breast tumor cells.

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