5-AMINOLEVULINIC ACID MEDIATED PHOTODYNAMIC THERAPY OF LEWIS LUNG CARCINOMA: A ROLE OF TUMOR INFILTRATION WITH DIFFERENT CELLS OF IMMUNE SYSTEM

Skivka Larisa M., Gorobets Oksana B., Kutsenok Vadim V., Lozinsky Miron O.

Aim: To investigate the effect of photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) as a photosensitizer on the character of infiltration of experimental tumors by different cells of immune system. Methods: The effect of ALA-PDT on subcutaneously implanted Lewis lung carcinoma in с57Вl/6 mice was studied. ALA at a dose of 500 mg/kg was given per os, and 4 h later tumors were subjected to laser irradiation (632 nm, 150 mW/cm2, 20 min). The evaluation of the tumor infiltration by myelomonocytic and lymphoid cells and oxygen-dependent metabolism of peritoneal macrophages (NBT-test) were carried out. Results: It is shown that ALA-PDT resulted in fast and massive infiltration of irradiated tumors by myelomonocytic cells, stimulation of the peritoneal macrophages metabolic activity and augmentation of the content of tumor infiltrating lymphocytes. Conclusion: The immunomodulation after ALA-PDT occurs via generation of inflammation and direct laser light activation of immune system cells.

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