CATALYTIC SYSTEM OF THE REACTIVE OXYGEN SPECIES ON THE C60 FULLERENE BASIS
Summary. Aim: To study the ability of fullerenes C60 to catalise the reactions of generation of reactive oxygen species (ROS) in water solution after photoexcitation and to affect the vitality of tumor cells in vitro. Methods: The number and vitality of cultured Ehrlich carcinoma cells or rat thymocytes were determined using tripane blue, ROS levels were registered using the methods of electron paramagnetic resonance (EPR) spectroscopy and spin traps, photoirradiation of water solution of fullerenes С60 with visible light was carried out using mercury lamp. Results: Irradiation of water solution of fullerenes С60 (10-5 М) was accompanied with generation of ROS with the rate of 10 nMol/ml/min. After addition of irradiated С60 solution to suspension of thymocytes or ascite cells, the decrease of the number of vital cells by 67 and 58%, respectively, has been registered. Conclusion: Photoirradiated water solutions of fullerenes С60 may be potentially useful for photodynamic therapy of tumors as ROS catalytic system.
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