2-[(3-CARBOXY-1-OXOPROGY1) AMINO]-2-DEOXY-D-GLUCOSE INDUCES APOPTOSIS IN Hep G2 CELLS

Wu Jing, Delu Qi, Kou Wei, Li Juan, Chen Xuan, Wang Aiqing, Liu Weiming, Xue Qunji, Qiao Liang

Aim: To determine the effect of 2-[(3-carboxy-1-oxoprogy1) amino]-2-deoxy-D-glucose (COPADG) on proliferation and apoptosis of human hepatocellular carcinoma cells (Hep G2). Methods: Hep G2 cells were cultured in RPMI-1640 medium in the presence of various concentrations of COPADG. Cell proliferation was determined by MTT assay. Apoptosis was determined by fluorescence microscopy, transmission electron microscopy, agarose gel electrophoresis of DNA fragmentation, and flow cytometry. Results: At the concentration ranging between 1–30 mM, COPADG potently inhibits growth and induce apoptosis of Hep G2 cells. Conclusions: COPADG could effectively induce apoptosis in Hep G2 cells. It may be potentially useful as a new agent for treatment of human hepatocellular carcinoma.

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