Expression of CD44 and Е-cadherin in the serous ovarian cancer and its clinical significance
N.Yu. Lukianova, O.D. Ryabtseva, L.Z. Polishchuk, V.F. Chekhun
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
Introduction: Molecules of cell adhesion (CD44 and E-cagherin) are significant for tumor growth and are connected to changes of cell proliferation and migration. Recently, it was hypothesized that cancer stem cells (CSCs) express CD44 which regulates cancer cell survival, metastatic potential, resistance to conventional radio-chemotherapy, disease relapse and prognosis. Aim: To evaluate the interrelations of CD44 and E-cadherin expression with clinical and cytomorphological features of serous ovarian cancer and its clinical significance. Patients and Methods: Immunohistochemical and morphological of CD44 and E-cadherin expression analysis was applied to pathological material obtained after surgery of 72 patients with ovarian cancer of I-III stage (FIGO classification) before neoadjuvant chemotherapy. Monoclonal antibodies against СD44 (clone DF 1485) and E-cagherin (clone NCH-38) were used. Results of immunohistochemical analysis were evaluated by semi-quantitative assessment, the percentage of CD44 (+) and presence/absence of E-cadherin (+) cells were estimated. Survival of patients was analyzed by Kaplan — Mayer method. Results: Variability in the number, distribution, and location of CD44 (+) and E-cadherin (+) tumor cells was observed in ovarian cancer patients. Expression of CD44 was defined in solid structures, and also in clusters of tumor cells (budding) located in stroma, in cells of tumor papilles, in adenomatous structures, and in gland lumen which in total provides the evidence of cellular discohesion and active invasion. The expression of CD44 (+) (over 10%) was observed in 58.3% patients. The negative correlation between CD44 (+) and E-cadherin (-) expression was determined (r = -0.38 p < 0.05). Survival analysis of ovarian cancer patients showed that 5-year survival of patients with tumor phenotype СD44 (+), Е-cadherin (-) was lower compared to survival of patients with tumor phenotype СD44 (-), Е-cadherin (+). Conclusion: In serous ovarian cancer variability of СD44 and E-cadherin expression was determined. СD44 expression in zones of cellular discohesion and active invasion of tumor cells was shown. We demonstrated the correlation between the percentage of CD44 (+) cells and survival of ovarian cancer patients. The results from this study suggest that quantification of CD44 (+) and E-cadherin (-) expression and their localization in ovarian cancer can be helpful as predictors of tumor progression.
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