Significance of COX-2 and VEGF expression in tumor for estimation of treatment results in cervical cancer stage IIB

Kryzhanivska A.Y.1, Andriiv A.V.1, Lukianova N.Yu.2

A.Y. Kryzhanivska1, A.V. Andriyiv1, N.Yu. Lukianova2

1Ivano-Frankivsk National Medical University,Ivano-Frankivsk, Ukraine

2R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine

anna.nivska@gmail.com

Introduction: One of the important tasks of modern oncogynecology is the estimation of biological characteristics of tumors. Among the biological markers studied in relation to cervical cancer, cyclooxygenase-2 (COX-2) and vascular endothelium growth factor (VEGF) play an important role in the processes of the tumor invasion and neoanginogenesis. Aim: To determine the COX-2 and VEGF expression in tumors and their connection with clinico-morphological indicators of the efficiency of treatment in patients with cervical cancer. Patients and Methods: Operation materials of 48 patients with cervical cancer stage IIB after chemotherapy was used. The immunohistochemical analysis of COX-2 and VEGF expression was performed using primary monoclonal antibodies (MCAB) of the “Diagnostic BioSystems” company, USA (clone 4H12) and “DakoCytomation”, Denmark (clone VG1) on the paraffin sections of the tumors. Results: A positive immunohistochemical reaction of specific MCAB to COX-2 was determined in 44 (92%) tumors, and to VEGF — in 42 (88%). It was shown that tumors with partial regression (PR), stabilization of the tumor process degree II of pathomorphosis after the treatment and the disease recurrence are characterized by high levels of the COX-2 and VEGF expression. Tumors with PR and with degree III of pathomorphosis after the treatment and disease recurrence are characterized by high levels of the COX-2 and VEGF expression but of low intensity. In patients with complete tumor regression after the treatment and without cervical cancer recurrence for more than 5 years no COX -2 and VEGF expressions were found. Conclusion: The results indicate that determining of the COX-2 and VEGF expression in tumor cells after treatment of patients could be considered as additional indexes for estimation of treatment efficiency and the prognosis of the cervical cancer course.

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