Ultrastructural and some functional changes in tumor cells treated with stabilized iron oxide nanoparticles

Yurchenko O.V., Todor I.N., Khayetsky I.K., Tregubova N.A., Lukianova N.Y., Chekhun V.F.

Aim: To study the ultrastructure and some functional indexes of tumor cells treated with stabilized iron nanoparticles in vitro. Methods: 3-[4,5dimethylthiazol-2-1]-2,5-diphenyltetrazolium bromide (MTT)-test, electron microscopy, polarography with applying of closed Clark’s electrode. Results: It was shown that cultivation of cells with stabilized Fe3O4 leads to intracellular accumulation of ferromagnetic nanoparticles. The most active ferromagnetic uptake by cells has been observed after 24 and 48 h of incubation. The presence of ferromagnetic in cells led to altered mitochondrial structure that caused the decrease of oxygen uptake rate in the cells of all studied lines. Ferromagnetic released from the majority of cells via exocytosis or clasmacytosis after a certain period of time. The number of dead cells or cells with severe damage was moderate, so cytotoxic action of stabilized iron oxide nanoparticles was minimal toward the studied cell lines. Conclusion: the presence of ferromagnetic nanoparticles in culture medium led to alterations in mitochondria ultrastructural organization and decrease of oxygen uptake by mitochondria in sensitive and anticancer-drugs resistant cells.

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