ANTISERA TO POLYCYCLIC AROMATIC HYDROCARBONS AND THEIR APPLICATION ON THE DETECTION OF CHEMICAL CARCINOGENS IN BLOOD SERA OF ONCOLOGICAL PATIENTS
Aim: To investigate the cross-reactivity of the hyperimmune antisera of animals (rabbits) and the sera of oncological patients to polycyclic aromatic hydrocarbons (PAHs) of a similar chemical structure. Methods: Reactions of antibodies with haptens have been estimated by non-competitive and competitive ELISA with synthesized protein conjugates of PAHs as a coating antigene. Results: All the model rabbit antisera have been stated to react with anthracene, chrysene, pyrene, benzo(a)pyrene and benz(a)anthracene irrespective of the hapten used to immunize animals. The cross-reactivity of serum antibodies to all five PAHs has been found in blood samples of oncological patients. Conclusion: It is recommended to use the conjugates of anthracene, chrysene and pyrene for detecting human antibodies to more dangerous environmental carcinogens — benzo(a)pyrene and benz(a)anthracene.
No Comments » Add comments