ANTISERA TO POLYCYCLIC AROMATIC HYDROCARBONS AND THEIR APPLICATION ON THE DETECTION OF CHEMICAL CARCINOGENS IN BLOOD SERA OF ONCOLOGICAL PATIENTS

Glushkov Andrey N., Anosova Tatiana P., Kostyanko Mikhail V., Anosov Mikhail P., Polenok Elena G., Cherno Sergey V., Mun Stella A.

Aim: To investigate the cross-reactivity of the hyperimmune antisera of animals (rabbits) and the sera of oncological patients to polycyclic aromatic hydrocarbons (PAHs) of a similar chemical structure. Methods: Reactions of antibodies with haptens have been estimated by non-competitive and competitive ELISA with synthesized protein conjugates of PAHs as a coating antigene. Results: All the model rabbit antisera have been stated to react with anthracene, chrysene, pyrene, benzo(a)pyrene and benz(a)anthracene irrespective of the hapten used to immunize animals. The cross-reactivity of serum antibodies to all five PAHs has been found in blood samples of oncological patients. Conclusion: It is recommended to use the conjugates of anthracene, chrysene and pyrene for detecting human antibodies to more dangerous environmental carcinogens — benzo(a)pyrene and benz(a)anthracene.

No Comments » Add comments
Leave a comment

ERROR: si-captcha.php plugin says GD image support not detected in PHP!

Contact your web host and ask them why GD image support is not enabled for PHP.

ERROR: si-captcha.php plugin says imagepng function not detected in PHP!

Contact your web host and ask them why imagepng function is not enabled for PHP.