EXPRESSION OF BETA-DEFENSIN-2 IN HUMAN GASTRIC TUMORS: A PILOT STUDY

Markeeva N.V.¹, Igor Lisovskiy², Valeriy Lyzogubov³, Vasilij Usenko⁴, Maria Soldatkina⁵, Serhey Merentsev⁶, Serhey Zaitsev⁷, Yuriy Kondratskii⁸, Anatoliy Tofan⁹, Serhey Osinskiy¹⁰, Petro Pogrebnoy¹¹

• ¹R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kyiv 03022, Ukraine
• ²Morphological Laboratory BIONTEC, Dnepropetrovsk, Ukraine
• ³Morphological Laboratory BIONTEC, Dnepropetrovsk, Ukraine
• ⁴Morphological Laboratory BIONTEC, Dnepropetrovsk, Ukraine
• ⁵Morphological Laboratory BIONTEC, Dnepropetrovsk, Ukraine
• ⁶Morphological Laboratory BIONTEC, Dnepropetrovsk, Ukraine
• ⁷Morphological Laboratory BIONTEC, Dnepropetrovsk, Ukraine
• ⁸Morphological Laboratory BIONTEC, Dnepropetrovsk, Ukraine
• ⁹Morphological Laboratory BIONTEC, Dnepropetrovsk, Ukraine
• ¹⁰Morphological Laboratory BIONTEC, Dnepropetrovsk, Ukraine
• ¹¹Morphological Laboratory BIONTEC, Dnepropetrovsk, Ukraine

Summary. The present work was aimed on the study of patterns of human beta-defensin-2 (hBD-2) expression in human gastric tumors. Materials and methods: 17 samples of surgically resected human gastric adenocarcinoma paired with respective normal tissue controls have been analyzed for the expression of hBD-2 by semi-quantitative RT-PCR and by immunohistochemical analysis using anti-hBD-2 monoclonal antibodies (mAbs) generated against recombinant hBD-2 by standard hybridoma technology. Results: The hyperexpression of hBD-2 on mRNA and protein levels was registered in 6 from 17 gastric tumor samples compared to respective controls; however, hBD-2 expression patterns seems not be related to the differentiation grade and stage of tumors and the presence of anti-Helicobacter pylori-antibodies in the blood serum of the patients. Conclusion: In human gastric adenocarcinomas expression of hBDs possesses heterogeneic character; the research on larger cohort of the patients with gastric cancer should be further performed.

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