THE ROLE OF EXPRESSION OF THE COMPONENTS OF PROTEOME IN THE FORMATION OF MOLECULAR PROFILE OF HUMAN OVARIAN CARCINOMA А2780 CELLS SENSITIVE AND RESISTANT TO CISPLATIN

Chekhun V.F., Lukyanova N.Yu., Urchenko O.V., Kulik G.I.

Aim: To study the expression of proteins that characterize drug resistance, proliferation and apoptosis of human ovarian carcinoma cells. Methods: The study was carried out on human ovarian carcinoma А2780 cells and on the A2780/DDP8 subline resistant to cisplatin. Expression of the surface and intracellular antigens (р53, Bcl-2, CD95, antigen of proliferating cells, metallothioneins, drug resistance proteins (P-glycoprotein (P-gp), glutathione-S-transferase), molecules of adhesion (Е-cadherin, a- and b-cate­nins) was studied by immunocytochemical method. Results: It has been shown that the formation оf the resistance to cisplatin in A2780/DDP8 cells is accompanied by the increase of expression of glutathione-S-transferase and Bcl-2, by the decrease of expression of CD95-antigene and proliferation potential of the cells, by appearance of EGF receptors and elevation of expression level of E-cadherin, a- and b-catenins, proving the enhancement of adhesive properties of tumor cells. Conclusion: Antiapoptotic program seems to be the leading mechanism of the development of the resistance to cisplatin in A2780/DDP8 cells and is realized via high expression of Bcl-2. During the development of drug resistance of A2780/DDP cells, the program of glutathione detoxification is functionally replacing the decrease of the content of metallothioneins.

No Comments » Add comments
Leave a comment

ERROR: si-captcha.php plugin says GD image support not detected in PHP!

Contact your web host and ask them why GD image support is not enabled for PHP.

ERROR: si-captcha.php plugin says imagepng function not detected in PHP!

Contact your web host and ask them why imagepng function is not enabled for PHP.