PRODUCTION OF RECOMBINANT OF hBD-2 — HUMAN ANTIMICROBIAL PEPTIDE EXPRESSED IN CERVICAL AND VULVAL CANCER

Pogribnoy P.V., Lisovskiy I.L., Markeeva N.V., Shnitsar V.M., Zinchenko I.I., Soldatkina M.A.

Summary. For preparation of recombinant human beta defensin-2 (hBD-2), the expression of the peptide fused with GST in bacterial system was chosen. hBD-2 cDNA fragment was cloned in pGEX-2T vector and the construct was introduced into E.coli XL-Gold cells. Fusion protein was purified by affine chromatography on GST-sepharose, and underwent proteolysis with trombin. Active recombinant hBD-2 was purified from the proteolytic mixture by the ion exchange chromatography.

No Comments » Add comments
Leave a comment

ERROR: si-captcha.php plugin says GD image support not detected in PHP!

Contact your web host and ask them why GD image support is not enabled for PHP.

ERROR: si-captcha.php plugin says imagepng function not detected in PHP!

Contact your web host and ask them why imagepng function is not enabled for PHP.