PRODUCTION OF RECOMBINANT OF hBD-2 — HUMAN ANTIMICROBIAL PEPTIDE EXPRESSED IN CERVICAL AND VULVAL CANCER
Summary. For preparation of recombinant human beta defensin-2 (hBD-2), the expression of the peptide fused with GST in bacterial system was chosen. hBD-2 cDNA fragment was cloned in pGEX-2T vector and the construct was introduced into E.coli XL-Gold cells. Fusion protein was purified by affine chromatography on GST-sepharose, and underwent proteolysis with trombin. Active recombinant hBD-2 was purified from the proteolytic mixture by the ion exchange chromatography.
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