MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF CHOLANGIOCELLULAR CARCINOMA RS-1 AFTER SUBCUTANEOUS AND INTRAHEPATIC TRANSPLANTATION

Treshalina H., Kobliakov V., Duzheva T., Raikhlin N., Andronova N., Sedakova L., Ostashkina N., Goloshapov R., Sorokina Yu., Gerasimova G.

Kinetics, histological and biochemical characteristics of transplantable cholangiocellular RS-1 carcinoma were studied after intrahepatic and subcutaneous grafting to outbred rats. Both grafts demonstrated similar growth patterns, i.e. slow growth with a 10 day latency and an exponential growth phase at days 18–24 following inoculation. Tumor growth rate was 10-fold lower and life span of tumor-bearing rats was longer after intrahepatic transplantation as compared to subcutaneous tumors. The tumors had identical histology (solid cancer with mucous areas). Cells in both tumors were characterized by the low mitotic activity, moderate rates of spontaneous apoptosis and necrosis. Biochemical characteristics in case of both routes of grafting were also identical, constitutive and benze(a)pyrene-inducible activities of glutathione-S-transferase, NAD(P)H-quinone oxidoreductase and aldehyde dehydrogenase in tumors being 17-fold lower than in the liver. NAD(P)H-quinone oxidoreductase and aldehyde dehydrogenase in hepatic tumors demonstrated the greatest fall in activity. RS-1 by the intrahepatic transplantation can be considered as a tumor growth model for regional administration of anticancer cytostatics.

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