ANTIBODIES TO TUMOR-ASSOCIATED CARBOHYDRATE EPITOPES IN SERA OF CANCER PATIENTS AND BLOOD DONORS

Smorodin E.P., Kurtenkov O.A., Sergeyev B.L., Lilleorg A.L., Chuzmarov V.I.

Enzyme-linked immunosorbent assay was applied for determination of antibodies to tumor-associated carbohydrate epitopes in serum of gastrointestinal and breast cancer patients and in blood donors. The synthetic polyacrylamide (PAA) conjugates with Thomsen–Friedenreich epitope (TF), TF-precursor (Tn), Gala1-3Galb (aGal), SiaTn, and SiaLea were used in the assay. Tris-PAA was used as a negative control. A significantly lower anti-TF and anti-Tn IgG levels were observed in gastric cancer patients in comparison with donors. In colorectal cancer patients a significantly lower anti-TF IgG levels were observed as well. In combined group of gastric and colorectal cancer patients at stage I of the disease a significantly lower anti-TF and anti-Tn IgG levels in comparison with donors were observed. Levels of anti-Tn IgG were significantly lower in donors with A blood-group phenotype than in B group. Anti-SiaTn and anti-SiaLea IgG antibodies in sera were revealed rarely. The negative correlation between anti-SiaLea IgG and CA 19–9 levels in serum of gastrointestinal cancer patients was observed: r = –0.4377, P = 0.047. Thus, gastrointestinal carcinoma is associated with low level of serum anti-TF IgG antibodies.

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