EXPRESSION OF DEFENSIN GENES IN A431 AND M-HeLa CELL LINES AND IN TUMORS OF HUMAN VULVAL AND CERVICAL CARCINOMA

Vinnitskaya A.B.1, Soldatkina M.A.2, Lisovskiy I.L., Nespryadko S.V.3, Lytvyn D.I., Markeeva N.V.4, Turchak O.V.1, Pogrebnoy P.V.2

Summary. The main purpose of the work was to study the patterns of defensin mRNA expression in epithelial cell lines A431 and M-HeLa originated from genital tumors, and in cells of human vulval and cervical carcinoma by quantitative RT-PCR. The expression of HNP1, HNP3, HP-4, Def5, HD6, hBD-1, hBD-2 genes has been studied. hBD-1 and HD-6 mRNA in A431 cells and HD-6 mRNA in M-HeLa cells were shown to be expressed constitutively whilst the expression of hBD-2 mRNA was induced by challenge of cells with B. subtilis or EGF. The investigation of the surgical specimens revealed the expression of hBD-2 mRNA which was absent in the normal tissue. hBD-1 mRNA in all samples of vulval and cervical carcinoma was overexpressed. The regularity in the expression patterns of HD-6 mRNA in the tumor and normal tissues was not found.

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