ANTIBODIES AGAINST DNA TOPOISOMERASE I IN BLOOD SERUM OF PATIENTS WITH PATHOLOGIES OF PANCREAS

Sidorik L.L., Todurov I.M., Vigontina O.G., Vudmaska M.I., Naydenov V.G., Kopchak V.M., Matsuka G.Ch.

DNA topoisomerase I (Topo I) as autoantigen has been analyzed in chronic pancreatitis. Topo I preparation from placenta of healthy women has been purified in three stages by chromatography. The enzymatic activity has been controlled in the reaction of supercoiled pBR322 relaxation. Topo I immunoreactivity has been assayed by ELISA and Western-blotting. The levels of anti-TOPO I autoantibodies in sera of 12 patients with chronic pancreatitis were shown to be higher than in healthy donors.

No Comments » Add comments
Leave a comment

ERROR: si-captcha.php plugin says GD image support not detected in PHP!

Contact your web host and ask them why GD image support is not enabled for PHP.

ERROR: si-captcha.php plugin says imagepng function not detected in PHP!

Contact your web host and ask them why imagepng function is not enabled for PHP.