APOPTOSIS INDUCTION IN HUMAN MALIGNANT LYMPHOID CELLS BY DNA-DAMAGING AGENTS WITH DIFFERENT MECHANISMS OF ACTION

Phylchenkov А.А., Zavelevich M.P., Butenko Z.A.

Induction of apoptosis by fludarabine phosphate and etoposide in human malignant lymphoid cell lines Namalwa, Raji, CEM, and MT-4 was analyzed on comparative basis using morphological criteria, flow cytometry and DNA electrophoresis in agarose gel. B cell line Namalwa was shown to be less susceptible to etoposide than to fludarabine. At the same time B cell line Namalwa was more resistant to etoposide than T cell line CEM. Fludarabine induced apoptosis both in B and T (MT-4) cells. While etoposide induced apoptosis in 4 h upon the beginning of incubation (short-term apoptosis), specific apoptotic changes induced by fludarabine were evident only in 72 h (delayed apoptosis). Preincubation of Namalwa or MT-4 cells with non-toxic concentration of zinc sulfate (40 mol/l) makes them more susceptible to apoptosis induction by etoposide and not by fludarabine being suggestive of zinc potentiation of short-term apoptosis in malignant lymphoid cells under study.

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